RP-UFLC Method Development and Validation for Simultaneous Estimation of Levofloxacin in Bulk and Tablet


International Journal of Pharmacy and Biomedical Engineering
© 2019 by SSRG - IJPBE Journal
Volume 6 Issue 1
Year of Publication : 2019
Authors : Pooja M, Sowmya H.G, Jose Gnana Babu C
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Pooja M, Sowmya H.G, Jose Gnana Babu C, "RP-UFLC Method Development and Validation for Simultaneous Estimation of Levofloxacin in Bulk and Tablet," SSRG International Journal of Pharmacy and Biomedical Engineering, vol. 6,  no. 1, pp. 46-53, 2019. Crossref, https://doi.org/10.14445/23942576/IJPBE-V6I1P107

Abstract:

A simple, novel, accurate, precise, linear, rapid, and economical UFLC method was developed to estimate Levofloxacin. The chromatographic separation was achieved using a Phenomenex Luna 5µ C18 (2) 100A (250 x 4.60mm 5 µ) column and binary gradient elution, a mobile phase comprising of Methanol: 0.3% Orthophosphoric acid (70: 30) at PH 1.66 was adjusted with water. The flow rate was 1.0 ml/min with detection at 294 nm using a UV detector and drug eluted with a retention time of 2.29 min. The calibration curves were linear (r²=0.999) in the concentration range of 0.2-1.0 μg/ml. The limit of detection and limit of quantitation was0.5931 and 1.7974μg/ml,respectively. Thus the simple, novel, economical, accurate, precise, and rapid UFLC method was developed to estimateLevofloxacin and validated as per ICH guidelines. Hence the method holds good for routine analysis of Levofloxacin in the pure and pharmaceutical dosage form.

Keywords:

- Levofloxacin, ICH guidelines, UFLC, Validation.

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