Genetic Diversity of Rosa Damascena Mill. in Latakia Province as Reveled by ISSR Analysis

International Journal of Agriculture & Environmental Science

© 2018 by SSRG - IJAES Journal

Volume 5 Issue 6

Year of Publication : 2018

Authors : Tharwat Redwan, Mazen Nassour, Hafez Mahfoud

10.14445/23942568/IJAES-V5I6P104

How to Cite?

Tharwat Redwan, Mazen Nassour, Hafez Mahfoud, "Genetic Diversity of Rosa Damascena Mill. in Latakia Province as Reveled by ISSR Analysis ," SSRG International Journal of Agriculture & Environmental Science, vol. 5,  no. 6, pp. 18-22, 2018. Crossref, https://doi.org/10.14445/23942568/IJAES-V5I6P104

Abstract:

The genetic relationships among 20 Rosa damascena Mill. accessions from various cultivation areas of Latakia province (Syria) and two accessions from type locality Al-Marah were analyzed using 10 ISSR primers. The ISSR primers produced 63 bands across the studied genotypes, of which 46 were polymorphic. The number of amplified bands varied from three to eight. The average numbers of bands per primer was 6.3 band. Percentage polymorphism ranged from 50% (ISSR-R9) to 100% (ISSR-D4, ISSR-R2), with an average percentage polymorphism of 73.02% across all the genotypes. Unweighted pair group method cluster analysis based on similarity values revealed 3 groups at the distance of dissimilarity 0.38. One includes 16 genotypes all from Latakia city, second cluster comprised all Gablah and Alkardaha genotypes, while Damascus genotypes (Al-Marah ) formed the third group and appeared to be distinct from all others. The wide genetic variation seen for R. damascena Mill. genotypes included in our study is a promising future for the breeding programs of rose.

Keywords:

Rosa damascena, Molecular characterization, ISSR, Cluster analysis

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