A Rapid Plant Production Technique of Aloe vera. L for Commercialization

International Journal of Agriculture & Environmental Science

© 2018 by SSRG - IJAES Journal

Volume 5 Issue 3

Year of Publication : 2018

Authors : Jugabrata Das, Sunil Bora

10.14445/23942568/IJAES-V5I3P106

How to Cite?

Jugabrata Das, Sunil Bora, " A Rapid Plant Production Technique of Aloe vera. L for Commercialization," SSRG International Journal of Agriculture & Environmental Science, vol. 5,  no. 3, pp. 30-37, 2018. Crossref, https://doi.org/10.14445/23942568/IJAES-V5I3P106

Abstract:

In recent times many techniques have been developed for mass production of high quality plants, amongst which In vitro regeneration of plants is widely used. Aloe vera which is frequently used as medicinal plant has a slow rate of growth mainly due to male sterility. Thus, In vitro culture of Aloe vera by using shoot tip as explant is used for commercial production. The process involves In vitro proliferation, In vitro rooting of the proliferated shoots and acclimatization of regenerated plantlets under ex vitro condition. The study on surface sterilization of explants revealed that 70% alcohol treatment for 1 minute followed by 0.10% HgCl2 for 2 minutes and ascorbic acid (1mg/l) for 10 minutes showed minimum microbial contamination without any toxicity to the explants. Explants gave the best response on medium containing 2.0 mg/l BAP + 0.5 mg/l NAA + 20.0 mg/l AdSO4, where greater number of explants (80%) were established in minimum duration (8 days). Multiple shoot proliferation and elongation were found to be the best on the medium supplemented with 4.0 mg/l BAP + 0.2 mg/l NAA + 20.0 mg/l AdSO4 in terms of maximum shoots per explants (23.8) with highest shoot length (4.22 cm). Highest rooting response (90%) with maximum roots per explants (10.8) having longest root length (6.8 cm) was found to be the best on medium supplemented with 2.0 mg/l IBA and 0.5 mg/l NAA. Plantlets with developed shoot and roots were then transferred for hardening and acclimatized under the greenhouse poly-tunnels where 90.00% of survival rate was achieved.

Keywords:

Aloe vera L., micro propagation, In vitro shoot induction, proliferation, rooting

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