Determination of a Group of Amino Acids in the Bioproteins Resulting from Bioremediation of Remains of Marine Life

International Journal of Agriculture & Environmental Science
© 2018 by SSRG - IJAES Journal
Volume 5 Issue 5
Year of Publication : 2018
Authors : Moufid Yassin, Izdihar Ammar, Badr Al Ali, Rabee Raya
How to Cite?

Moufid Yassin, Izdihar Ammar, Badr Al Ali, Rabee Raya, "Determination of a Group of Amino Acids in the Bioproteins Resulting from Bioremediation of Remains of Marine Life ," SSRG International Journal of Agriculture & Environmental Science, vol. 5,  no. 5, pp. 32-37, 2018. Crossref,


The research was conducted by isolating some micro organisms from the marine environment, and using it in remediation the remains of marine life. These organisms were characterized by their ability to secrete a disintegrated enzymatic spectrum of compounds in these residues and to build their protein-rich biomass through their metabolism. The results of the study showed the highest percentage of total protein in fermented biomass by Micrococcus variants which reached to (42.11%). An increase in the ratio of amino acids in this mass was notable, especially lysine (7.01 g / 100 g bioprotein) and methionine (1.96 g / 100 g bioprotein). A comparison between the protein produced from these organisms in the present study and the traditional vegetable and animal protein was performed. It was observed that the ratios of the amino acids studied were higher than those in the vegetable protein, which allowed it to be a real protein substitute in economic animals feed


Bioprotein, bioremediation and amino acids


[1] UPADHYAYA,S.;TIWARI,S.AND ARORA, N.K. SINGHD.P. Microbial Protein: A Valuable Component for Future Food Security.Microbes and Environmental Management.vol. 10, 2016, 260-278.
[2] GOUR, S.; NUPUR,M.; ANURADHA,S. AND PRADEEP,B. Single Cell Protein Production: A Review . International Journal of Current Microbiology and Applied Sciences. vol. 4, No. 9,2015,251-262.
[3] AHMADI,A.R.; GHOORCHIAN,H.;HAJIHOSAINI,R.; and KHANIFAR,J. Determination of the Amount of Protein and Amino Acids Extracted from the Microbical Protein (SCP) of Lignocellulosic Wastes. Pakistan Journal of Biological Sciences. Vol. 13, No.8, 2010, 355-361.
[4] NASSERI, A.T.; RASOUL-AMINI,S.; MOROWVAT, M.H. AND GHASEMI, M.H. Single Cell Protein: Production and Process. American Journal of Food Technology. Vol. 6, 2011, 103-116.
[5] KANDASAMY,K ALIKUNHI,N.M AND SUBRAMANIAN,M. Yeasts in marine and estuarine environments. Journal of Yeast and Fungal Research Vol. 3(6), pp. 74 – 82, .2012.
[6] SIBTAIN, A.; MUSTAFA,G.; ARSHAD,M. AND RAJOKA,M. Fungal Biomass Protein Production fromTrichodermaharzianum Using Rice Polishing. BioMed Research International. Vol.1, 2017, 1-9.
[7] SINGH,A.; ABIDI, A. B .; AGRAWAL. A. K. AND. DARMWAL, N. S. Single Cell Protein Production by Aspergillusniger and Its Evaluation. Zentralbl. Mikrobiol. 146 (1991), 181-184.
[8] MOHAMED, N. E. ; EL-SAYED, E. A.; YOUNIS, N. A. AND HASAN, I. A. Biological Evaluation of Single Cell Protein..Journal of Radiation Research and Applied Sciences. Vol. 4, No. 2, 691 – 707 ,2011.
[9] KHAN,Y. M.;. UMAR DAHOT, M .; AHMED NAQVI.S. Amino Acids Composition Of Mycelial Protein Of EnicilliumExpansum Grown In Acid Treated Rice Husk Mineral Medium. Pak. J. Bot., 44(3): 1159-1165, 2012.
[10] TESFAW,A. AND ASSEFA,F. co-culture: Agreat promising method in single cell protein production . Biotechnology and molecular Biology reviews. vol. 9, No. 2, 2014,12-20 .
[11] SIBI,G.;DHANANJAYAK.;RAVIKUMAR,K.;MALLESHA,H.;VENKATESHA,T.; TRIVEDI,D.; BHUSAL,K.; AND GOWDA,K . Preparation of Glucosamine Hydrochloride from Crustacean Shell Waste and It’s Quantitation by RP-HPLC American-Eurasian Journal of Scientific Research. 8 (2), 2013, 63-67.
[12] KAM,S.; KENARI, A.; AND YOUNESI.H. Production of Single Cell Protein in Stickwater by Lactobacillus acidophilus and Aspergillusniger. Journal of Aquatic Food Product Technology, 21:403–417, 2012.
[13] AOAC. Official Methods of Analysis. Association of Official Analytical Chemists. 2005.
[14] QASSIM, B. B and HAMEED, S. F Anovel approach for the spectrophotometric determination of tryptophanin drug samples of different origins using homemade FIA/ merging zones techniques .Analytical Chemistry An Indian Journal. 16(11) 2016 [464-474].
[15] BASHEIR, B. E. A.; and ELBASHIR, A. A. Spectrophotometric methods for the determination of L-tyrosine in pharmaceutical formulations. Chem Xpress.Vol. 8, No. 2, 2015, 95-101.
[16] DUHANYNA,I.V.; AYRAPETOVA,A. J.; and LAZARYAN.G.D. Quantification of determination of amino acids in pollen Hym. - farm. Zh. - 2006. – Vol. 40, No.2, - P. 22-23
[17] LOGOYDA, L. S.; VRONSKA, L. V. ; DEMID, A. E.; MYKHALKIV M. M. Spectrophotometric determination of glycine in tablets containing glycine and some plant extracts . Pharmaceutical Journal. - 2010. - № 4. - P. 50-54.
[18] FERREL R.E.; FELLERS ,D.A. and SHEPHERO,A.D. determination of free lysine methionine in amino acid-fortified wheat .Agricultural Research USA.Vol615.1969.
[19] HAMDY, H. S. Production of mini-food by Aspergillusniger, Rhizopusoryzae and Saccharomyces cerevisiae using orange peels. Romanian Biotechnological Letters Vol. 18, No.1, 2013.
[20] CARRERA,S. C.; REYNOSO, C. M.; FUNES, G. J.; MARTÍNEZ. M. J.; DARDANELL, I. J.; and RESNIK, S. L. Amino acid composition of soybean seeds as affected by climatic variables.Pesq. agropec. bras., Brasília, Vol. 46, No. 12, 2011, 1579-1587.
[21] ADEYEYE. E.I.The Comparison of the Amino Acids Profiles of Whole Eggs, Francolin and Turkey Consumed in Nigeria .Global Journal of Science Frontier Research Chemistry .Vol. 13, No. 3, 2013.
[22] CHI,S.; TAN,B.; LIN,H.; MAI,K.; AI,Q.; WANG,X.; ZANG,W.; XU,W. and LIUFU,Z. Effects of supplementation of crystalline or coated methionine on growth performance and feedutilization of the Pacific white shrimp, Litopenaeusvannamei. Aquacult. Nutr.Vol.17, No. 2, 2011, 2095.2009.
[23] L´OPEZ-CERVANTES, J.; S´ANCHEZ-MACHADO, D. I. and ROSAS-RODR´IGUEZ, J.A.Analysis of free amino acids in fermented shrimp waste by high-performance liquid chromatography.Journal of Chromatography A, 1105,2006,106–110.
[24] SEPTINOVA, D.; KURTINI, T. and TANTALO, S. Evaluation the Usage of Treated Shrimp Waste as Protein Source in Broiler Diet. Animal Production 12 vol. 1, 2012, 1-5.
[25] WHO/FAO/UNU Expert Consultation. Energy and protein requirements. Geneva: World Health Organ Tech Rep.; 724:264 pages, 1985.
[26] WHO/FAO/UNU Expert Consultation. Proteins and amino acids in human nutrition. World Health Org Tech Rep Ser.; 935:265 pages, 2007.