Genetic Relationship among Salvia Species in the Syrian Coast Assessed by ISSR and RAPD Markers

International Journal of Agriculture & Environmental Science

© 2019 by SSRG - IJAES Journal

Volume 6 Issue 1

Year of Publication : 2019

Authors : DimahAli Zreikah, AbdAllah Barakat, AmmarAmran

10.14445/23942568/IJAES-V6I1P102

How to Cite?

DimahAli Zreikah, AbdAllah Barakat, AmmarAmran, "Genetic Relationship among Salvia Species in the Syrian Coast Assessed by ISSR and RAPD Markers," SSRG International Journal of Agriculture & Environmental Science, vol. 6,  no. 1, pp. 12-17, 2019. Crossref, https://doi.org/10.14445/23942568/IJAES-V6I1P102

Abstract:

The genus Salvia includes an enormous assemblage of nearly 1000 species dispersed around the World. Most medicinal plants like Salvia have originated from wild herbs, so information on their genetic characters is essential for their conservation and domestication. Hence, it is important to evaluate the genetic diversity of Salvia in Syria. ISSR and RAPD markers were used to evaluate the genetic relationship among fifteen ecotypes of seven Salvia species collected from different locations of the Syrian Coast (Lattakia and Tartous). Amplification of genomic DNA using 28 primers (14 RAPD and 14 ISSR) produced 133 bands, of which 114 were polymorphic (85.7%). The Nei’s dissimilarity index varied from 0.07 to 0.40 for RAPD and from 0.12 to 0.52 for ISSR data. The dendrogram was constructed using UPGMA method with bootstrapping 100 times using the iTOL software V.2.3, which distinguished four main clusters among seven species of Salvia based on RAPD and ISSR analyses, respectively. It was generally observed that both ISSR and RAPD markers had similar efficiency in detecting genetic polymorphisms but ISSR wasmore reproducible than RAPD with remarkable ability to differentiate the closely related ecotypes of Salvia. Based on the results of clustering and AMOVA, the genetic diversity among the species was confirmed. Furthermore, our genetic diversity analysis could provide useful information for utilization of these species, especially for genetic improvement.

Keywords:

Salvia, genetic relationship, RAPD, ISSR, dendrogram, polymorphism, clustering, AMOVA

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