In Vitro Antioxidant Potentials and total Phenolic Content of Tunisian Datura Metel Extracts from Leaves and Seeds

International Journal of Agriculture & Environmental Science

© 2019 by SSRG - IJAES Journal

Volume 6 Issue 1

Year of Publication : 2019

Authors : Leyla Tahrani, Fathia Harzallah-Skhiri, Mahjoub Aouni and Karima Bel Hadj Salah

10.14445/23942568/IJAES-V6I1P104

How to Cite?

Leyla Tahrani, Fathia Harzallah-Skhiri, Mahjoub Aouni and Karima Bel Hadj Salah, "In Vitro Antioxidant Potentials and total Phenolic Content of Tunisian Datura Metel Extracts from Leaves and Seeds," SSRG International Journal of Agriculture & Environmental Science, vol. 6,  no. 1, pp. 24-28, 2019. Crossref, https://doi.org/10.14445/23942568/IJAES-V6I1P104

Abstract:

The objective of this study is to determine the antioxidant activity and total phenols of Datura metel, a medicinal plant collected in Tunisia. Leaves and seeds of the plant were subject to cold maceration with solvent extracts: petroleum ether (PE), dichloromethane (CH2Cl2), ethyl acetate (ACOEt) and methanol (MeOH). Total phenolic contents were investigated by the method of Folin-Ciocalteu. Antioxidant activities of different extracts were determined by DPPH radical scavenging, ABTS assay and reducing power. All tested D. metel extracts showed interesting antioxidant activities and a difference was observed between leaves and seeds antioxidant potential extracts. Our results showed that the methanolic seeds extracts exhibited the highest total phenolic content. At 1 mg/ml concentration, the DPPH radical scavenging activities of leaves and seeds were 66.4% and 63.3% respectively, and the inhibition percentages of ABTS radical were between 96.54% and 97.01%. Our results suggest that D. metel extracts from both leaves and seeds could be used as potential sources of new antioxidant agents, useful in pharmacological and food industries.

Keywords:

Datura metel, antioxidant activity, total phenolic content, seeds, leaves.

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