Synthesis and Characterization of Solanum Nigrum Derived Nanoparticles and Exploration if Its Antioxidant, Antibacterial And Anticancer Potentials in in Vitro

International Journal of Agriculture & Environmental Science

© 2019 by SSRG - IJAES Journal

Volume 6 Issue 1

Year of Publication : 2019

Authors : Rajalakshmi. A, Sreelatha. S and Jayachitra. A

10.14445/23942568/IJAES-V6I1P105

How to Cite?

Rajalakshmi. A, Sreelatha. S and Jayachitra. A, "Synthesis and Characterization of Solanum Nigrum Derived Nanoparticles and Exploration if Its Antioxidant, Antibacterial And Anticancer Potentials in in Vitro," SSRG International Journal of Agriculture & Environmental Science, vol. 6,  no. 1, pp. 29-36, 2019. Crossref, https://doi.org/10.14445/23942568/IJAES-V6I1P105

Abstract:

In recent years research in the synthesis of nanoparticles were investigated, for their exhibit in larger surface area, thus opening to many possibilities with respect to all technological applications. The Solanum nigrum leaf extracts were used to synthesize silver nanoparticles, which was confirmed by various spectral datas. The maximum absorption in UV spectrum for silver nanoparticle formation was at 458nm, the SEM image clearly indicates the shape of silver nanoparticles. The EDAX spectrum showed the presence of silver signal. All the characterization results revealed the synthesized silver nanoparticles size was between 100nm. After confirmation of silver nanoparticles, they were analyzed for its scavenging activity against free radicals. The synthesised silver nanoparticles were experimentally proved by its antibacterial activity using Well diffusion method. Further, to evaluate the cytotoxicity of silver nanoparticles in in vitro model, AGS (colon cancer cell line) and Hep-2 (Larynx cancer cell line) were used and analysed for its cell cycle arrest using Flow cytometer.

Keywords:

Solanum nigrum, silver nanoparticles, SEM, DPPH, Hep2cell line , AGS cell line, MTT and FACS

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