Molecular Characterization of Several Isolates of Entomopathogenic Nematode Heterorhabditisbacteriophora Poinar (Rhabditida, Heterorhabditidae) from Soil of Citrus Orchards in Latakia, Syria

International Journal of Agriculture & Environmental Science

© 2019 by SSRG - IJAES Journal

Volume 6 Issue 2

Year of Publication : 2019

Authors : Ghadah Zeini, Nabil AboKaf, Mazen Al- Body, Majda Mofleh

10.14445/23942568/IJAES-V6I2P113

How to Cite?

Ghadah Zeini, Nabil AboKaf, Mazen Al- Body, Majda Mofleh, "Molecular Characterization of Several Isolates of Entomopathogenic Nematode Heterorhabditisbacteriophora Poinar (Rhabditida, Heterorhabditidae) from Soil of Citrus Orchards in Latakia, Syria," SSRG International Journal of Agriculture & Environmental Science, vol. 6,  no. 2, pp. 70-74, 2019. Crossref, https://doi.org/10.14445/23942568/IJAES-V6I2P113

Abstract:

Heterorhabditisbacteriophora was isolated from the soil of citrus orchards in different localities of Latakia Governorate, Syria. Collected material was identified based on the morphometric and morphological characters. Internal transcribed spacer 1 (ITS1) of five isolates was amplified using the PCR. Sequences of collected material have compared with available genetic data in the GeneBank. The identity of the collected material with the available sequence reached up to 99%. Phylogeny tree of the isolates inferred based on the ITS1 sequences of five Syrian isolates and some international sequences of the same and other related species using Steinernemafeltiae as an outgroup. Syrian isolates of H. bacteriophora grouped in the same clad with other related isolates of neighboring countries.

Keywords:

Entomopathogenic, Heterorhabditis, ITS1, Isolate, Syria.

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