Simple And Rapid Technique For Measure of Liquid Paints Antimicrobial Activity

International Journal of Agriculture & Environmental Science

© 2019 by SSRG - IJAES Journal

Volume 6 Issue 5

Year of Publication : 2019

Authors : Małgorzata Robak, Tomasz Koźlecki

10.14445/23942568/IJAES-V6I5P113

How to Cite?

Małgorzata Robak, Tomasz Koźlecki, "Simple And Rapid Technique For Measure of Liquid Paints Antimicrobial Activity," SSRG International Journal of Agriculture & Environmental Science, vol. 6,  no. 5, pp. 60-64, 2019. Crossref, https://doi.org/10.14445/23942568/IJAES-V6I5P113

Abstract:

Microbial invasion of building materials, including paints, is a manufacturer and future habitants problem. So, a need of antimicrobials protected materials, especially paints, is crucial for health of peoples and animals. The way of testing this antimicrobial activity in different kinds of paints is not always easy. The viscosity of emulsion and oil-paints makes the traditional microbiological technique very difficult or impossible. We propose to make serial decimal dilutions of bacteria and fungi suspension, performed a night incubation (10-16 hours) at room temperature with tested twice diluted paint(s), then special inoculation on agar medium (5 μL), incubation for 24-48 hours at 25-30°C and counting of developed colonies. Not killed by paint microorganisms have been going to growth as colony on agar. The technique was tested on four different emulsion paints and the results were clear and easy to interpret. Using this technique biocidal activity of silver nanoparticles at concentration of 6.25 mg/L was confirmed. This methodology could be applied for all liquid products involved in building materials, house cleaning and body hygiene.

Keywords:

biocidal activity, silver nanoparticles, AgNPs, paint

References:

[1] E. Englebert, G.T. Kleinheinz and B.M. Langolf, ―Characterization of airborne fungal levels after mold remediation,‖ Microbiological Research, 2006. vol.161, pp.367-376.
[2] A. Gniadek, A. Macura, E. Krajewska-Kułak and E. Oksiejczuk, ―Fungi in the air of selected social welfare homes in the Małopolskie and Podlaskie provinces–a comparative study,‖ International Bioteroration & Biodegradation: 2005, vol 5, pp.85-91.
[3] A.A. Haleem Khan and S. Mohan Karuppayil, ―Fungal pollution of indoor environments and its management,‖ Saudi J Biol Sci. 2012, vol.19(4), pp. 405–426.
[4] C.R. Łukaszuk, E. Krajewska-Kułak and W. Kułak, ―Effects of fungal air pollution on human health,‖ Prog. Health Sci. 2011, vol. 1(2), pp. 156-164.
[5] N. Duran, M. Duran, M.B. de Jesus, A.B. Seabra, W.J. Favaro and G. Nakazano, ―Silver nanoparticles : a new view on mechanistic aspects on antimicrobial activity,‖ Nanomed NBM, 2016, vol.12, pp.789-799.
[6] M. Guzman, J. Dille and S. Guzman, ―Synthesis and antibacterial activity of silver nanoparticles against grampositive and gram-negative bacteria,‖ Nanomed NBM, 2012, vol. 8, pp. 37-45.
[7] W-R. Li, X-B. Xie, Q-S. Shi, H-Y Zeng, Y-S. OU-Yang and Y-B. Chen, ―Antibacterial activity and mechanism of silver nanoparticles on Escherichia coli,‖ Appl. Microbiol. Biotechnol. 2010, vol. 85, pp. 1115-1122.
[8] P. Nowicka-Krawczy, J. Żelazna-Wieczorek and T. Koźlecki, ―Silver nanoparticles as a control agent against facades coated by aerial algae—A model study of Apatococcus lobatus (green algae),‖ PLoS ONE 2017, vol. 12(8): e0183276. https://doi.org/10.1371/journal.pone.0183276
[9] J. Pulit, M. Banach, R. Szczygłowska and M. Bryk,‖Nanosilver against fungi. Silver nanoparticles as an effective biocidal factor,‖ Acta Biochim. Pol. 2013, vol. 60(4), pp. 795-798.
[10] M, Rai A, Yadav and A. Gade,‖Silver nanoparticles as a new generation of antimicrobials,‖ Biotechnol. Adv. 2009, vol. 27, pp. 76-83.
[11] Q.H, Tran V.Q. Nguyen and A-T. Le, ‖Silver nanoparticles: synthesis, properties, toxicology, applications and perspectives,‖ Adv. Nat. Sci.: Nanosci. Nanotechnol. 2013, DOI: 10.1088/2043-6262/4/3/033001.
[12] M.A. Dar, A. Ingle and M. Rai, ―Enhanced antimicrobial activity of silver nanoparticles synthesized by Cryphonectria sp. evaluated singly and in combination with antibiotics,‖ Nanomed. Nanotechnol. Biol. Med. 2013,vol. 9, pp.105-110.
[13] I. Sondi and B. Salopek-Sondi, ―Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for gram-negative bacteria,‖ J. Colloid Interf. Sci. 2004, vol.275, pp. 177-182.
[14] K-J. Kim, W.S. Sung, B.K. Suh, S-K. Moon, J-S. Choi, J.G. Kim and D.G. Lee, ―Antifungal activity and mode of action of silver nano-particles on Candida albicans,” Biometals. 2009, vol. 22, pp. 235-242.
[15] B. Żarowska, M. Piegza, K. Jaros-Koźlecka, T. Koźlecki and M. Robak, ―Antimicrobial activity of silver nanoparticles,‖ in proceedings of international conference: BRIA-Biotechnology, Research and Industrial Applications, Wrocław, Poland, September, 2015, pp 55.
[16] GB/T 1741-2007, ―Test method for determining the resistance of paints film to mold,‖ Ed. Chinese National Standard, 2007.
[17] PN-EN ISO 846, 2002. ―Tworzywa sztuczne. Ocena działania mikroorganizmów,‖ Ed. Polski Komitet Normalizacyjny, 2002.
[18] T. Koźlecki, H. Teterycz, A. Sokołowski, I. Polowczyk, W. Sawiński, I. Maliszewska and J. Szydło, ―Sposób syntezowania nanocząstek srebra,‖ PL Patent deposition No P 395979, August 2011.
[19] B. Żarowska, T. Kożlecki, M. Piegza, K. Jaros-Koźlecka, and M. Robak, ―New look on antifungal activity of silver nanoparticles (AgNPs)‖ Polish Journal of Microbiology,‖ 2019, (in press)
[20] B. Żarowska, M. Piegza, K. Jaros-Koźlecka, T. Koźlecki and M. Robak,. ―Nanoczastki srebra (SNP) a hamowanie wzrostu mikroorganizmów,‖ in Nowoczesne Technologie Produkcji Żywności (ISBN 978-83-7160-837-7), 2016, pp. 36-48.
[21] A. Koziróg, K. Rajkowska, A. Otlewska, M. Piotrowska, A. Kunicka-Styczynska, B. Brycki, P. Nowicka-Krawczyk, M. Koscielniak and B. Gutarowska, ―Protection of historical wood against microbial degradation—selection and application of microbiocides,‖ Int. J. Mol. Sci. 2016,
vol..17, pp. 1364, DOI:10.3390/ijms17081364
[22] S. Kokura, O. Handa, T. Takagi, T. Ishikawa, Y. Naito and T.Yoshikawa, ―Silver nanoparticles as a safe preservative for use in cosmetics,‖ Nanomedicine, Nanotechnology, Biology, and Medicine, 2010, vol.6, pp. 570-574.
[23] K.B. Riaz Ahmed, A.M. Nagy, R.P. Brown, Q. Zhang, S.G. Malghan and P.L.Goering, ―Silver nanoparticles: significance of physicochemical properties and assay interference on the interpretation of in vitro cytotoxicity studies,‖ Toxicol. in Vitro 2017,vol. 38, pp. 179-192.
[24] J-P. Kaiser, M. Roesselin, L. Diener, A. Wichser, B. Nowack and P. Wick, ―Cytotoxic effects of nanosilver are highly dependent on the chloride concentration and the presence of organic compounds in the cell culture media,‖ J Nanobiotechnol. 2017,vol 15, pp. 5.
[25] T, Sandle ed.The CDC Handbook: A Guide to Cleaning and Disinfecting Clean rooms (1st ed.). Grosvenor House Publishing Limited. 2012. ISBN 978-1781487686