Laboratory Study of the Effect of Methanolic and Water Extracts of Aromatic Plant Seeds in the Growth of the Two Pathogenic Fungi Fusarium oxysporum and Aspergillus niger

International Journal of Agriculture & Environmental Science
© 2016 by SSRG - IJAES Journal
Volume 3 Issue 6
Year of Publication : 2016
Authors : Somar Shaban, Nawal Ali
How to Cite?

Somar Shaban, Nawal Ali, "Laboratory Study of the Effect of Methanolic and Water Extracts of Aromatic Plant Seeds in the Growth of the Two Pathogenic Fungi Fusarium oxysporum and Aspergillus niger," SSRG International Journal of Agriculture & Environmental Science, vol. 3,  no. 6, pp. 42-46, 2016. Crossref,


A Study was carried out to evaluate the ability of the methanolic and water extractions of anise seeds (Pimpinella anisum L.) to inhibit the growth of the pathogenic fungi Fusarium oxysporum and Aspergillus niger. The results showed a good inhibition ability of the methanolic and water extractions on the growth of the studied fungi. The inhibition ability differed due to the used concentrations. When the methanolic extraction was used by concentrations (2, 4, and 6) mlg/ ml at the seventh day, the inhibition ability was (42.5, 50.6 and 64.4)% of the fungus F. oxysporum, respectively. And was (23.6, 42.7 and 55.1)% of the fungus A. niger, respectively. Whereas when the water extraction was used by the same concentrations, the inhibition ability was (59.2, 65.3 and 70)% respectively for the fungus F. oxysporum, and (46.1, 58 and 61)% for the fungus A. niger, respectively.



Pimpinella anisum Fusarium oxysporum Aspergillus niger, Methanolic extraction, and water extraction.


1) R.A. Humber, "Evolution of entomopathogenicity in fungi". Journal of Invertebrate pathology. 98: 262- 266. 2008.
2) L.A. Lacey, "Manual of Techniques in invertebrate pathology". Second edition. USA. Academic Press. Pp 484. 2012.
3) T.M. Butt, C. Jackson and N. Magan. "Fungi as biocontrol agents, Progress, problems and potential." CABI publishing, UK, pp. 390. 2001.
4) S. Keller, and G. Zimmerman. "Mycopathogens of soil insects. In: Wilding, N; N.M. Collins; P.M. Hammond and J.F. Webber, (Eds.), Insect-Fungus Interactions." Academic Press, London, UK, pp. 240-270. 1989.
5) A.E. Hajek, "Ecology of terrestrial fungal entomopathogens." Adv. Microb. Ecol. 15, 193–249. 1997.
6) G.D. Inglis, M.S. Goettel; T.M. Butt and H. Strasser. "Use of Hyphomycetous Fungi for managing insect pestes. In: Butt. T.M; C.Jackson and N. Magan, (Eds.). Fungi as biocontrol agents, Progress, problems and potential". CABI publishing, UK, PP: 23-69. 2001.
7) N.V. Meyling, and J. Eilenberg. "Ecology of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae in temperate agroecosystems: Potential for conservation biological control". Biological Control 43:145–155. 2007.
8) H.E. Roy,; D.C. Steinkraus, J. Eilenberg, A.E. Hajek and J.K. Pell, "Bizarre interactions and endgames: Entomopathogenic fungi and their arthropod hosts". Annual Review of Entomology, 51: 331-357. 2006.
9) W.G. Meikle, S.T. Jaronski, G. Mercadier and P.C. Quimby. "A distributed delay routine-based simulation model of Beauveria bassiana conidial stability in response to environmental stressors". Biocontrol 48, 561–578. 2003.
10) M.S. Goettel, G.D. Inglis, and S.P. Wraight, "Fungi. In: Field Manual of Techniques in Invertebrate Pathology. L. A. Lacey and H. K. Kaya", Eds. Kluwer Academic Publishers, Netherlands. pp. 255-282. 2000a.
11) D.W. Roberts, "World picture of biological control of insects by fungi". Mem. Inst. Oswaldo Crus, Rio de Janeiro, vol. 84, 89- 100. 1989.
12) G.D. Inglis, J. Enkerli and M.S. Goettel, "Laboratory techniques used for entomopathogenic fungi: Hypocreales. In: Lacey. L.A., (Eds.). Manual of Techniques in invertebrate pathology". Second edition. USA. Academic Press. pp 189-253. 2012.
13) J. Fargues, N.K. Maniania, J.C. Dekmas and N. Smiths, "Influence de la temperature sur la croissance In vitro d´hyphomycetes entomopathogenes." Agronomie, 12: 557-564. 1992.
14) H. Yeo.; J.K. Pell, P.G. Alderson, S.J. Clark and B.J. Pye. "Laboratory evaluation of temperature effects on the germination and growth of entomopathogenic fungi and on their pathogenicity to two aphid species". Pest Manag Sci 59:156–165. 2003.
15) V.H. Kreisel, and F. Schauer, "Methoden des mykologischen laboratoriums". Fisher verlag Jena, Germany, pp181. 1987.
16) S.A, Rehner, F. Posada, E.P. Buckley, F. Infante, A. Castillo and F.E Vega. "Phylogenetic origins of African and Neotropical Beauveria bassiana s.l. pathogens of the coffee berry borer, Hypothenemus hampei." J. Invertebr. Pathol. 93, 11–21. 2006.
17) J.D. Walstad,; R.F. Anderson and W.J. Stambaugh. 1970. Effect of environmental conditions on two species of muscardin fungi (Beauveria bassiana and Meterhizium anisopliae). J. Invertebrate Pathology, 2: 221- 226.
18) J.E. Hallsworth, and N. Magan, "Water and temperature relations of growth of the entomopathogenic fungi Beauvaria bassiana, Meterhizium anisopliae and Paecilomyces farinosus." J. Invertebrate Pathology, 74: 261-266. 1999.
19) E. Quesada- Moraga, E.A.A. Maranhao, P. Valverde- Garcia and C. Santiago- Alvarez, "Selection of Beauveria bassiana isolates for control of the whiteflies Bemisia tabaci and Trialeurodes vaporariorum on the basis of their virulence, thermal requirements, and toxicogenic activity". Biological Control 36: 274- 287. 2006.
20) P. Kessler, H. Matzke and S. Keller, "The effect of application time and soil factors on the occurrence of Beauveria brongniartii applied as a biological control agent in soil". J. Invertebr. Pathol. 84, 15–23. 2003.
21) P. Cheong, "Bioactive Metabolites of an Isolate of Entomopathogenic Fungus Beauveria bassiana." Lincoln University. A thesis submitted in partial fulfillment of the requirements for the Degree of Doctor of Philosophy. pp 281. 2015.
22) H.D. Burges, "Microbial Control of Pests and Plant Diseases 1970-1980." Academic pres INK. (LONDON) Ltd., PP 949. 1981.
23) A.L. Nussenbaum, M. A. Lewylle and R. E. Lecuona. "Germination, Radial Growth and Virulence to Boll Weevil of Entomopathogenic Fungi at Different Temperatures." World Applied Sciences Journal, 25 (8): 1134-1140. 2013.
24) I.C.C. Hsia, M.T. Islam, Y. Ibrahim, T.Y. Howand and D. Omar, "Evaluation of conidial viability of entomopathogenic fungi as influenced by temperature and additive". Int. J. Agric. Biol., 16: 146‒ 152. 2014.
25) J.S. Kim, M. Skinner, S. Gouli and B.L. Parker. "Generating thermotolerant colonies by pairing Beauveria bassiana isolates". FEMS Microbiol Lett 324: 165- 172. 2011.
26) R. S. Soper, and M. G. Ward. "Production formulation and application of fungi of insect control. In: Papaviza, G.C. (Ed.), Biological control in crop production". Allanheld and Osmun, Totowa, pp. 161- 180. 1981.
27) D.W. Roberts, and A. W. Sweeney. "Production of fungi imperfect with vector control protection. In: Invertebrate pathology and microbial control". Proceeding 3rd international Colloquium on Invertebrate pathology, University of Sussex, pp: 409- 413. 1982.
28) M.M. Sabbour,; M. Ragei and A. Abd-El Rahman. "Effect of Some Ecological Factors on The Growth of Beauveria bassiana and Paecilomyces fumosoroseus Against Corn Borers." Australian Journal of Basic and Applied Sciences, 5(11): 228-235. 2011.
29) W. Petlamul, and P. Prasertsan. "Evaluation of Strains of Metarhizium anisopliae and Beauveria bassiana against Spodoptera litura on the Basis of Their Virulence, Germination Rate, Conidia Production, Radial Growth and Enzyme Activity." Mycobiology 40(2) : 111-116. 2012.
30) S.A. Safavi,; F. A. Shah, A. K. Pakdel, G. R. Rasoulian, A. R. Bandani and T. M. Butt. "Effect of nutrition on growth and virulence of the entomopathogenic fungus Beauveria bassiana." FEMS Microbiol Lett 270: 116– 123. 2007.
31) M. El- Damir, "Effect of growing media and water volume on conidial production of Beauveria bassina and Metarhizuim anisopliae." Journal of biological sciences 6(2): 269- 274. 2006.
32) T.A. Ugine,; S.P. Wraight, M. Brownbridge and J.P. Sanderson. "Development of a novel bioassay for estimation of media lethal concentrations (LC50) and doses (LD50) of the entomopathogenic fungus Beauveria bassiana, against western flower thrips, Frankliniella occidentalis." J Invertebr Pathol 89: 210–218. 2005.