Description of the cultivable bacterioma of the digestive tract and gills of Oreochromis niloticus and isolation of bacteria with probiotic potential

International Journal of Agriculture & Environmental Science
© 2020 by SSRG - IJAES Journal
Volume 7 Issue 5
Year of Publication : 2020
Authors : MOROH Jean-Luc Aboya, KARAMOKO Detto, KOKORA Aya Philomène, ANGOUA Amanahan Mauricette Prisca, YAO Ange Olivier Parfait, DAGO Dougba Noël, COULIBALY Adama
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MOROH Jean-Luc Aboya, KARAMOKO Detto, KOKORA Aya Philomène, ANGOUA Amanahan Mauricette Prisca, YAO Ange Olivier Parfait, DAGO Dougba Noël, COULIBALY Adama, "Description of the cultivable bacterioma of the digestive tract and gills of Oreochromis niloticus and isolation of bacteria with probiotic potential," SSRG International Journal of Agriculture & Environmental Science, vol. 7,  no. 5, pp. 14-20, 2020. Crossref, https://doi.org/10.14445/23942568/IJAES-V7I5P103

Abstract:

The use of antibiotics in aquaculture has led to multiple problems, the most worrying of which worldwide is the emergence of resistance from pathogenic bacteria. In this context, the use of probiotics in aquaculture is presented as an alternative to antibiotics. This work aims to isolate bacteria with probiotic potential from the gills and the digestive tract of Tilapia niloticus usable in aquaculture. Its implementation first calls for the enumeration and description of the total bacterioma and the two organs' lactic bacterioma. Then, to evaluate the antagonistic activities of the bacteria described vis-à-vis the pathogenic strains Escherichia coli and Staphylococcus aureus by Fleming's method.
The work carried out on wild (Bandama river) and farmed (fish farm) Tilapia has made it possible to isolate 206 bacterial strains (non-demanding lactic flora and total flora) from the gills and digestive tract of this fish. The microbial load is higher in the gills than in the digestive tract in all of the fish sampled. Antimicrobial activity tests revealed 35 strains with probiotic potential, including five strains with antagonistic activity against E. coli and S. aureus simultaneously, 8 strains with antagonistic activity against E. coli against 27 strains with anti-S. Aureus activity. These strains with probiotic potential come mainly from the digestive tract. Furthermore, these strains with probiotic potential are higher in wild fish than in farmed fish. The 35 strains of interest isolated after further studies could be used as a probiotic in aquaculture.

Keywords:

Oreochromis niloticus, probiotics, bacteriome, aquaculture

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