Deleterious Rhizobacteria As A Potential Bioherbicide-A Review

International Journal of Agriculture & Environmental Science
© 2021 by SSRG - IJAES Journal
Volume 8 Issue 2
Year of Publication : 2021
Authors : Jimni Phukan, Jayanta Deka, Khagen Kurmi, Sontara Kalita
How to Cite?

Jimni Phukan, Jayanta Deka, Khagen Kurmi, Sontara Kalita, "Deleterious Rhizobacteria As A Potential Bioherbicide-A Review," SSRG International Journal of Agriculture & Environmental Science, vol. 8,  no. 2, pp. 1-5, 2021. Crossref,


Weed is a serious problem in crop production as it competes with the crop for essential growth factors and results in remarkable yield losses. Conventionally, many agronomic practices have been adopted for weed management, but they are less efficient, expensive, and laborious. Chemical herbicides are effective, but their long-term repeated use may cause weed resistance and serious environmental pollution. Considering all the secondary effects and environmental impact of herbicides, the future of weed management is to rely on alternative approaches such as the biological method of weed control. One such upcoming biological approach to control weed is the use of deleterious rhizobacteria (DRB). DRB is reported to suppress the weed dynamics providing scope for the crop to compete with the suppressed weeds for the essential growth requirements. This review focuses on the potentiality of DRB to be used as a bioherbicide.


bioherbicide, deleterious rhizobacteria, phytotoxin, Pseudomonas, weed


[1] Berner, D.K., Schaad, N.W. and Volksh. B. Use of ethylene-producing bacteria for stimulation of Striga spp. Seed germination. Biological Control. 15 (1999) 274-282.
[2] Campbell, J.N., Corm, K., Sorlie, L. and Cook, F.D. Inhibition of growth in canola seedlings caused by an opportunistic Pseudomonas sp. under laboratory and field conditions. Canadian Journal of Microbiology. 32(1986) 201-207.
[3] Cleyet-Marel, J.C., Larcher, M., Bertrand, H., Rapior, S. and Pinochet, X. Plant growth enhancement by rhizobacteria. EdIn Morot-Gaudry, J-F. (Ed), Nitrogen Assimilation by Plants: Physiological, Biochemical, and Molecular Aspects. (2001) 185-197.
[4] Dan, H. A., Dan, L. G. M., Barroso, A. L. L., Procópio, S. O., Oliveira Jr, R. S., Silva, A. G., Lima, M.D.B. and Feldkircher, C. Residual activity of herbicides used in soybean agriculture on grain sorghum crop succession. Planta Daninha. 28(2010) 1087-1095.
[5] Fett, W.F., Osman, S.F. and Dunn, M.F. Characterization of exopolysaccharides produced by plant-associated fluorescent pseudomonads. Applied & Environmental Microbiology. 55 (1989) 579–583.
[6] Floresā€Vargas, R. and O'hara, G. Isolation and characterization of rhizosphere bacteria with potential for biological control of weeds in vineyards. Journal of applied microbiology. 100(2006) 946-954.
[7] Fujimori, T. New developments in plant pathology in Japan. Australasian Plant Pathology. 28(1999) 292-297.
[8] Gealy, D.R.S., Gurusiddaiaah, G.O.GG., and Ogg Jr, A.G. Isolation and characterization of metabolites from Pseudomonas syringea- strain 3366 and their phytotoxicity against certain weed and crop species. Weed Science. 44(1996) 383-392.
[9] Gerhardson, B., Alstrom, S. and Ramert, B. Plant reactions to inoculation of roots with fungi and bacteria. PhytopathologischeZietschrift. 114(1985) 108-117.
[10] Gurusiddaiah, S., Gealy, D.R., Kennedy, A.C. and Ogg, A.G., Jr. Isolation and characterization of metabolites from Pseudomonas fluorescens D7 for control of downy brome (Bromus tectorum). Weed Science. 42(1994) 492-501.
[11] Heap, I. Herbicide-resistant weeds. In Integrated pest management. Springer, Dordrecht. (2014) 281-301.
[12] Howie, W.J. and Echandi, E. Rhizobacteria: influence of cultivar and soil type on plant growth and yield of potato. Soil Biology and Biochemistry. 15(1983) 127-132.
[13] Husain, A. and Kelman, A. Relation of slime production to the mechanism of wilting and pathogenicity of Pseudomonas solanacearum. Phytopathology. 48(1958) 155–165.
[14] Imaizumi, S., Nishino, T., Miyabe, K., Fujimori, T. and Yamada, M. Biological Control of Annual Bluegrass (Poa annua L.) with a Japanese Isolate of Xanthomonas campestris pv. poae (JT-P482). Biological control. 8(1997) 7-14.
[15] Jones, D.L., Farrar, J. and Giller, K.E. Associative nitrogen fixation and root exudation – what is theoretically possible in the rhizosphere? Symbiosis. 35 (2003) 19-38.
[16] Kelman, A. The relationship of pathogenicity in Pseudomonas solanacearum to colony appearance on a tetrazolium chloride medium. Phytopathology. 44(1954) 693–695.
[17] Kennedy, A. C., and Kremer, R. J. Microorganisms in weed control strategies. Journal of Production Agriculture. 9(4)(1996) 480-485.
[18] Kennedy, A., Young, F., Elliott, L. and Douglas, C. Rhizobacteria suppressive to the weed downy brome. Soil Science Society of America Journal. 55 (1991) 722-727.
[19] Knowles, C.J. and Bunch, A.W. Microbial cyanide metabolism. Advances in Microbiol Physiology. 27 (1986) 73-111.
[20] Kremer, R. J. Deleterious rhizobacteria. In Plant-Associated Bacteria. Springer, Dordrecht. (2006) 335-357.
[21] Kremer, R. J. Growth suppression of annual weeds by deleterious rhizobacteria integrated with cover crops. In Proceedings of the Xth International Symposium on Biocontrol of Weeds. Bozeman, MT, USA: Montana State University. (2000) 931-940.
[22] Kremer, R.J., Caesar, A.J. and Souissi, T. Soilborne microorganisms of Euphorbia are potential biological control agents of the invasive weed leafy spurge. Applied Soil Ecology. 32(1) (2006) 27–37.
[23] Kremer, R.J. and Kennedy, A.C. Rhizobacteria as biocontrol agents of weeds. Weed Technology. 10 (1996) 601-609.
[24] Kremer, R.J. and Souissi, T. Cyanide production by rhizobacteria and potential for suppression of weed seedling growth. Current Microbiology. 43 (2001) 182–186.
[25] Lakshmi, V., Kumari, S., Singh, A. and Prabha, C. Isolation and characterization of deleterious Pseudomonas aeruginosa KC1 from rhizospheric soils and its interaction with weed seedlings. Journal of King Saud University-Science. 27(2) (2014) 113-119.
[26] Li, J. and Kremer, R. J. Growth response of weed and crop seedlings to deleterious rhizobacteria. Biological Control. 39(1) (2006) 58-65.
[27] Li, J., Kremer, R.J. and Ross, L.M., Jr. Electron microscopy of root colonization of Setaria viridis by deleterious rhizobacteria as affected by soil properties. Symbiosis. 32 (2002) 1-14.
[28] Liddell, C. M., and Parke, J. L. Enhanced colonization of pea taproots by a fluorescent pseudomonad biocontrol agent by water infiltration into the soil. Phytopathology. 79(12)(1989) 1327-1332.
[29] Lindow, S.E. and Brandl, M.T. Microbiology of the phyllosphere. Applied and Environmental Microbiology. 69 (2003) 1875-1883.
[30] Loper, J. E., and Buyer, J. S. Siderophores in microbial interactions on plant surfaces. Molecular Plant-Microbe Interactions. 4(1)(1991) 5-13.
[31] Loper, J. E., Haack, C. and Schroth, M. N. Population dynamics of soil pseudomonads in the rhizosphere of potato (Solanum tuberosum L.). Applied and Environmental Microbiology. 49(2) (1985) 416-422.
[32] Mazzola, M., Stahlman, P. W. and Leach, J. E. Application method affects the distribution and efficacy of rhizobacteria suppressive of downy brome (Bromus tectorum). Soil Biology and Biochemistry. 27(10)(1995) 1271-1278.
[33] McPhail, K. L., Armstrong, D. J., Azevedo, M. D., Banowetz, G. M. and Mills, D. I. 4-Formylaminooxyvinylglycine, an herbicidal germination-arrest factor from Pseudomonas rhizosphere bacteria. Journal of natural products. 73(11)(2010) 1853-1857.
[34] Miché, L., Bouillant, M. L., Rohr, R., Sallé, G. and Bally, R. Physiological and cytological studies on the inhibition of Striga seed germination by the plant growth-promoting bacterium Azospirillum brasilense. European Journal of Plant Pathology. 106(4)(2000) 347-351.
[35] Nehl, D., Allen, S. and Brown, J. Deleterious rhizosphere bacteria: an integrating perspective. Applied Soil Ecology. 5 (1997) 1-20.
[36] Owen, A. and Zdor, R. Effect of cyanogenic rhizobacteria on the growth of velvetleaf (Abutilon theophrasti) and corn (Zea mays) in
autoclaved soil and the influence of supplemental glycine. Soil Biology & Biochemistry. 33(2001) 801–809.
[37] Patil, V. S. Rhizospheric bacteria with the potential for biological control of Parthenium hysterophorus. Journal of Chemical, Biological and Physical Sciences: 3(4)(2013) 2679.-2686.
[38] Persello-Cartieaux, F., Nussaume, L. and Robaglia, C. Tales from the underground: molecular plant-rhizobacteria interactions. Plant Cell Environ. 26(2003) 186-199.
[39] Rao, V.S. Principles of weed science. Second edition, published by Mohan Primlani for Oxford and IBH Publishing Co. Pvt. Ltd., New Delhi, (2000) 1
[40] Sarwar, M. and Kremer, R. J. Enhanced suppression of plant growth through the production of L-tryptophan-derived compounds by deleterious rhizobacteria. Plant and Soil. 172(2) (1995) 261-269.
[41] Schippers, A.B., Bakker, A.W. and Bakker, P.A.H.M. Interactions of deleterious and beneficial rhizosphere microorganisms and the effect of cropping practices. Annual Review Phytopathology. 25 (1987) 339-358.
[42] Schippers, B., Bakker, A. W., Bakker, P. A. H. M. and Van Peer, R. Beneficial and deleterious effects of HCN-producing pseudomonads on rhizosphere interactions. Plant and Soil. 129(1) (1990) 75-83.
[43] Schroth, M.N. and Hancock, J.G. Disease-suppressive soil and root-colonizing bacteria. Science. 216(1982) 1376–1381.
[44] Shirdashtzadeh, M. A. R. Y. A. M. Deleterious rhizobacteria as weed biological control agent: development and constraints. Asian Journal of Microbiology, Biotechnology, and Environmental Sciences. 16(3)(2014) 561-574.
[45] Souissi, T., Kremer, R.J. and White, J.A. Scanning and transmission electron microscopy of root colonization of leafy spurge (Euphorbia esula L.) seedlings by rhizobacteria. Phytomorphology. 47(1997) 177-193.
[46] Stubbs, T. L., and Kennedy, A. C. Microbial weed control and microbial herbicides. Herbicides—Environmental impact studies and management approaches. InTech, Rijeka, Croatia. (2012) 135-166.
[47] Suckstorff, I. and Berg, G. Evidence for dose-dependent effects on plant growth by Stenotrophomonas strains from different origins. Journal of Applied Microbiology. 95 (2003) 656–663.
[48] Tranel, P. J., Gealy, D. R. and Kennedy, A. C. Inhibition of downy brome (Bromus tectorum) root growth by a phytotoxin from Pseudomonas fluorescens strain D7. Weed Technology. (1993) 134-139.
[49] Xie, H., Pasternak, J.J. and Glick, B.R. Isolation and characterization of mutants of the plant growth-promoting rhizobacterium Pseudomonas putida GR12-2 that overproduce indoleacetic acid. Current Microbiology. 32 (1996) 67–71.
[50] Vessey, J.K. Plant growth-promoting rhizobacteria as biofertilizers. Plant Soil. 255 (2003) 571-586.
[51] Vilich, V. and Sikora, R.A. Diversity in soilborne microbial communities. In Boland, G.J. & Kuykendall, L.D. (Eds), Plant-Microbe Interactions and Biological Control. Marcel Dekker: New York. (1998) 1-14.
[52] Zeller, S. L., Brandl, H., and Schmid, B. Host-plant selectivity of rhizobacteria in a crop/weed model system. PLoS One. 2(9) (2007) 846.
[53] Zdor, R. E., Alexander, C. M. and Kremer, R. J. Weed suppression by deleterious rhizobacteria is affected by formulation and soil properties. Communications in soil science and plant analysis. 36(9-10) (2005) 1289-1299.
[54] Mansoor Ahmad Bhat and Yogamoorthi A, Allelopathic Influence of Tecomastans(L.) on the Seed Germination and Biochemical Changes in Green Gram., SSRG International Journal of Agriculture & Environmental Science 5(5)(2018) 38-48.