The Assessment of Genetic Polymorphism in Triticale SC1 Somaclones using RAPD-PCR Markers

International Journal of Agriculture & Environmental Science

© 2023 by SSRG - IJAES Journal

Volume 10 Issue 1

Year of Publication : 2023

Authors : Renata Ciobanu

10.14445/23942568/IJAES-V10I1P102

How to Cite?

Renata Ciobanu, "The Assessment of Genetic Polymorphism in Triticale SC1 Somaclones using RAPD-PCR Markers," SSRG International Journal of Agriculture & Environmental Science, vol. 10,  no. 1, pp. 9-17, 2023. Crossref, https://doi.org/10.14445/23942568/IJAES-V10I1P102

Abstract:

The triticale varieties are becoming more and more widespread crops as human and animal feed. To carry out genotyping of the valuable varieties, molecular markers are important in identification and polymorphism analysis. In the proposed investigation, triticale somaclones derived from three triticale cultivars (Ingen 35, Ingen 93, 188TR5021) were evaluated and compared to the forms obtained from seeds exposed to gamma irradiation (150 Gy). Molecular genetic variability was evaluated based on twelve arbitrary primers using the RAPD method. The results of genotyping with RAPD primers found the most informative oligomers in discriminating the triticale forms. According to the higher weight showed a pronounced polymorphism between somaclones, irradiated and recurrent cultivars. A higher genetic diversity has been identified in forms derived from cultivar Ingen 93 (25% polymorphism) compared to Ingen 35 (15%) or 188TR5027 (19%), especially in somaclone obtained from Ingen 93, confirmed by the number of specific fragments (5) and the highest dissimilarity index (0.26). The significant variability in the frame of somaclones was confirmed by the genetic distance based on the Jaccard index, differentiating them from the control and irradiated treatments.

Keywords:

Triticale, Genetic polymorphism, RAPD primers, Somaclone.

References:

[1] Badea et al., “Development and Assessment of DArT Markers in Triticale,” Theoretical and Applied Genetics, vol. 122, pp. 1547-1560, 2011. Crossref, https://doi.org/10.1007/s00122-011-1554-3
[2] Y.V. Chesnokov, and A.M. Artemyeva, “Evalution of polymorphism information of genetic diversity,” Agricultural Biology, vol. 50, no. 5, pp. 571-578, 2015.
[3] Karen Cichy, and Blair J. Goates, “Evaluation of Molecular Markers for Common Bunt Resistance Genes in Diverse Wheat Genotypes,” Abstract In: ASA-CSSA-SSA International Annual Meetings, 2009.
[4] Esra Ayodogan Cifci, and Koksal Yagdi, “Study of Genetic Diversity in Wheat (Triticum Aestivum) Varieties using Random Amplified Polymorphic DNA (RAPD) Analysis,” Turkish Journal of Field Crops, vol. 17, no. 1, pp. 91-95, 2012.
[5] DimahAli Zreikah, AbdAllah Barakat, and AmmarAmran, “Genetic Relationship among Salvia Species in the Syrian Coast Assessed by ISSR and RAPD Markers,” SSRG International Journal of Agriculture & Environmental Science, vol. 6, no.1, p.12-17, 2019. Crossref, https://doi.org/10.14445/23942568/IJAES-V6I1P102
[6] Nagwa Kh. El-Safty, and Mohamed A. Attia, “Genetical and Productivity Evaluation of Some Triticale Genotypes Under North Sinai Conditions,” Egyptian Journal of Desert Research, vol. 69, no. 2, pp. 107-125, 2019. Crossref, https://doi.org/10.21608/ejdr.2021.19433.1067
[7] S. Ganea, L. Cota, and C. Botez, „RAPD and SSR Marker Selection for Some Dihaploid Wheat Lines for Septoria Tritici Resistance,” Journal of Horticulture, Forestry and Biotehnology, vol. 13, pp. 114-117, 2009.
[8] F. A. Hafashjani, “Genetic Variation of Iranian Iris Species using Morphological Characteristics and RAPD Markers,” International Journal of AgriScience, vol. 2, no. 9, pp. 875-889, 2012.
[9] Anna Kalinka, and Magdalena Achrem, “Reorganization of Wheat and Rye Genomes in Octoploid Triticale,” Planta, vol. 247, no. 4, pp. 807-829, 2018. Crossref, https://doi.org/10.1007%2Fs00425-017-2827-0
[10] A.G.A. Khaled, M.H. Motawea, and A.A. Said, “Identification of ISSR and RAPD Markers Linked to Yield Traits in Bread Wheat Under Normal and Drought Conditions,” Journal of Genetic Engineering and Biotechnology, vol. 13, no. 2, pp. 243-252, 2015. Crossref, https://doi.org/10.1016/j.jgeb.2015.05.001
[11] Pradeep Kumar et al., “Potential of Molecular Markers in Plant Biotechnology,” Plant Omics Journal, vol. 2, no. 4, pp. 141-162, 2009.
[12] Jianna Machczyńska, Janusz Zimny, and Piotr Tomasz Bednarek, “Tissue Culture-induced Genetic and Epigenetic Variation in Triticale (x Triticosecale spp. Wittmack ex A. Camus 1927) Regenerants,” Plant Molecular Biology, vol. 89, no. 3, pp. 279-292, 2015. Crossref, https://doi.org/10.1007/s11103-015-0368-0
[13] A. Mayavel et al., “RAPD Polymorphism and Genetic Distance Among Phenotypic Variants of Tamarindus Indica,” Journal of Korean Society of Forest Science, vol. 109, no. 4, pp. 421-428, 2020. Crossref, https://doi.org/10.14578/jkfs.2020.109.4.421
[14] Pawel Milczarski, Aneta Banek-Tabor, and Piotr Masojć, “Wykorzystanie Markerów RAPD do Identyfikacji Odmian Pszenżyta [Application of RAPD Markers for Fingerprinting of Triticale Cultivars],” Biulletin of Plant Breeding Acclimatization Institute, pp. 261- 266, 2001.
[15] M. G. Murray, and W. F. Thompson, “Rapid Isolation of High Molecular Weight Plant DNA,” Nucleic Acids Research, vol. 8, no. 19, pp. 4321-4325, 1980. Crossref, https://doi.org/10.1093%2Fnar%2F8.19.4321
[16] O. A. Orlovskaya, L, V. Koren, and L. V. Khotyleva, “Genetic Polymorphism Evaluation of Spring Triticale (x Triticosecale Wittmack) Samples with Use of RAPD and ISSR Markers,” Russian Journal of Genetics: Applied Research, vol. 2, pp. 508-512, 2012. Crossref, http://dx.doi.org/10.1134/s207905971206010x
[17] Aparna Priyadarshini Patra, Arup Kumar Mukherjee, and Laxmikant Acharya, “Comparative Study of RAPD and ISSR Markers to Assess the Genetic Diversity of Betel Vine (Piper betle L.) in Orissia, India,” American Journal of Biochemistry and Molecular Biology, vol. 1, no. 2, pp. 200-211, 2011. Crossref, https://dx.doi.org/10.3923/ajbmb.2011.200.211
[18] Lenka Petrovičová et al., “Assessment of RAPD Polymorphism in Rye (Secale cereale L.) Genotypes,” Journal of Microbiology, Biotechnology and Food Science, vol. 4, no. 2, pp. 94-97, 2015. Crossref, http://dx.doi.org/10.15414/jmbfs.2015.4.special2.94-97
[19] J. Sambrook, and D.W. Russell, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, New York, vol. 2, 3rd edn., 2001.
[20] Ajay Sharma et al., “Molecular Markers: New Prospects in Plant Genome Analysis,” Pharmacognosy Reviews, vol. 2, no. 3, pp. 23-34, 2008.
[21] Izabela Szućko, and Anna Mądrach, “Application of ITAP-PCR Techniques to Assess the Genetic Variability of Selected Cultivars of Winter Triticale (× Triticosecale Wittmack),” Notulae Botanicae Horti Agrobotanici Cluj-Napoca, vol. 47, no. 3, pp. 947-953, 2019. Crossref, https://doi.org/10.15835/nbha47311494
[22] F. Tonk et al., “Evalutaion and Comparison of ISSR and RAPD Markers for Assessment of Genetic Diversity in Triticale Genotypes,” Bulgarian Journal of Agricultural Science, vol. 20, no. 6, pp. 1413-1420, 2014.
[23] Andrej Trebichalský et al., “Detection of Genetic Diversity of Triticale by Microsatellite Markers,” Journal of Microbiology, Biotechnology and Food Sciences, vol. 2, no. 1, pp. 1898–1906, 2013.
[24] E. Veveriţă, and P. Buiucli, “Rolul Materialului Iniţial în Procesul de Creare a Formelor Noi de Triticale Hexaploide Secundare,” Agricultura Moldovei, vol. 5, no. 6, pp. 23-26, 2008.
[25] J. G. K. Williams et al., “DNA Polymorphisms Amplified by Arbitrary Primers are Useful as Genetic markers,” Nucleic Acids Research, vol. 18, no. 22, pp. 6531-6535, 1990. Crossref, https://doi.org/10.1093/nar/18.22.6531
[26] P. Winter, and G. Kahl, “Molecular Marker Technologies for Plant Improvement,” World Journal of Microbiology and Biotechnology, vol. 11, no. 4, pp. 438-448, 1995. Crossref, https://doi.org/10.1007/bf00364619