Nutritional and Sensory Characteristics of Steamed Brownies When Substituting Wheat Flour (Triticum aestivum L.) with Oyster Mushroom Flour (Pleuiotus ostreatus)

International Journal of Agriculture & Environmental Science

© 2025 by SSRG - IJAES Journal

Volume 12 Issue 1

Year of Publication : 2025

Authors : Tatang Sopandi, Fifi Alaida Putri, Wardah

10.14445/23942568/IJAES-V12I1P104

How to Cite?

Tatang Sopandi, Fifi Alaida Putri, Wardah, "Nutritional and Sensory Characteristics of Steamed Brownies When Substituting Wheat Flour (Triticum aestivum L.) with Oyster Mushroom Flour (Pleuiotus ostreatus)," SSRG International Journal of Agriculture & Environmental Science, vol. 12,  no. 1, pp. 28-37, 2025. Crossref, https://doi.org/10.14445/23942568/IJAES-V12I1P104

Abstract:

Consumer behaviour that wants healthy food encourages producers to design products with better nutritional composition and health benefits. Wheat flour, as the main ingredient for bakery products, lacks lysine, has high gluten content associated with celiac disease, and has a high glycemic index. This current study aims to evaluate the content of protein, fat, carbohydrates, and crude fiber as well as the overall properties of sensory, color, taste, aroma, and texture of steamed brownies due to the substitution of wheat flour (Triticum aestivum L.) with oyster mushroom flour (Pleurotus ostreatus) in the dough formula. The current study was conducted using a completely randomized design with 5 treatments in the proportion of substituting wheat flour with oyster mushroom flour: 0%, 5%, 10%, 15%, and 20% in the dough formulation for making steamed brownies, each of which was repeated 5 times. The study's findings indicated that the substitution of wheat flour with 5-20% oyster mushroom flour considerably (p<0.05) increased the crude protein, crude fat, and crude fiber content while considerably (p<0.05) decreasing the carbohydrate content of steamed brownies. Substituting wheat flour with 5-20% oyster mushroom flour in the dough formula also increased the panelists' acceptance of the overall properties, color, taste, and texture, but at a proportion of 20%, it decreased their acceptance of the aroma of steamed brownies. The current study concluded that wheat flour could be substituted with 15% oyster mushroom flour in the dough formulation for making steamed brownies.

Keywords:

Nutrition, Oyster mushrooms, Sensory, Steam brownies, Wheat.

References:

[1] Malnutrition, World Health Organization, 2019. [Online]. Available: https://www.who.int/news-room/fact-sheets/detail/malnutrition
[2] Nur Fitri Widya Astuti, Emy Huriyati, and Susetyowati Susetyowati, “Prevalence and Factors Associated with the Occurrence of Double Nutritional Burden in Families in Indonesia,” The Indonesian Journal of Public Health, vol. 16, no. 1, pp. 100-115, 2020.
[CrossRef] [Google Scholar] [Publisher Link]
[3] Natisha Dukhi, Global Prevalence of Malnutrition: Evidence from Literature, Malnutrition, pp. 1-16, 2020.
[CrossRef] [Google Scholar] [Publisher Link]
[4] Gargi Ghoshal, and Prerna Kaushik, “Development of Soymeal-Fortified Cookies to Combat Malnutrition,” Legume Science, vol. 2, no. 3, pp. 1-13, 2020.
[CrossRef] [Google Scholar] [Publisher Link]
[5] Ali A. Yousaf et al., “Nutritional and Functional Evaluation of Wheat Flour Cookies Supplemented with Gram Flour,” International Journal of Food Science and Nutrition, vol. 64, no. 1, pp. 63-68, 2013.
[CrossRef] [Google Scholar] [Publisher Link]
[6] Abimbola K. Arise, Ganiyat O. Taiwo, and Sunday A. Malomo, “Amino Acid Profile, Pasting, and Sensory Properties of Croissant Snacks Produced from Wheat-Fermented Bambara Flour,” Legume Science, vol. 2, no. 4, pp. 1-9, 2020.
[CrossRef] [Google Scholar] [Publisher Link]
[7] Masood Sadiq Butt, and Rizwana Batool, “Nutritional and Functional Properties of Some Promising Legumes Proteins Isolates,” Pakistani Journal of Nutrition, vol. 9, no. 4, pp. 373-379, 2010.
[CrossRef] [Google Scholar] [Publisher Link]
[8] Yuan Biao et al., “Impact of Mushroom (Pleurotus Eryngii) Flour upon Quality Attributes of Wheat Dough and Functional Cookies-Baked Products,” Food Science and Nutrition, vol. 8, no. 1, pp. 361-370, 2019.
[CrossRef] [Google Scholar] [Publisher Link]
[9] Center for Agricultural Data and Information System, Statistics of Food Consumption, 2020. [Online]. Available: https://satudata.pertanian.go.id/assets/docs/publikasi/Statistik_Konsumsi_Pangan_Tahun_2020.pdf
[10] Indra Agustanugraha Pramadi et al., “Proportion of MOCAF and Arrowroot Flour with Addition of Maltodextrin on Cookies Processing,” Journal of Agrotechnology, vol. 13, no. 2, pp. 137-147, 2019.
[Google Scholar]
[11] Pawan Kumar et al., “Nutritional Contents and Medicinal Properties of Wheat: A Review,” Life Sciences and Medicine Research, vol. 22, no. 1, pp. 1-10. 2011.
[Google Scholar]
[12] Paulo Duarte, Mariana Teixeira, and Susana Costa e Silva, “Healthy Eating as a Trend: Consumers’ Perceptions towards Products with Nutrition and Health Claims,” Brazilian Business Management Journal, vol. 23, no. 3, pp. 405-421, 2021.
[CrossRef] [Google Scholar] [Publisher Link]
[13] F.M.N.A. Aida et al., “Mushroom as a Potential Source Prebiotics: A Review,” Trends in Food Science and Technology, vol. 20, no. 11 12, pp. 565-575, 2009.
[CrossRef] [Google Scholar] [Publisher Link]
[14] S.M. Nahid Hasan, and Md. Nazmul Aunsary, “Impact of Supplanting of Flour with Mushroom Powder on Nutritional Composition and Sensory Attributes of Cookies,” Malaysian Journal of Halal Research, vol. 3, no. 2, pp. 43-49, 2020.
[CrossRef] [Google Scholar] [Publisher Link]
[15] Biao Yuan et al., “In Vitro and in Vivo Inhibitory Effects of a Pleurotus Eryngii Protein on Colon Cancer Cells,” Food & Function, vol. 8, no. 10, pp. 3553-3562, 2017.
[CrossRef] [Google Scholar] [Publisher Link]
[16] Jung Eun-Kyun, and Joo Na-Mi, “Optimization of Iced Cookie Prepared with Dried Oak Mushroom (Lentinus Edodes) Powder Using Response Surface Methodology,” Korean Society of Food and Cookery Science, vol. 26, no. 2, pp. 121-128, 2010.
[Google Scholar] [Publisher Link]
[17] Enkhjargal Ulziijargal et al., “Quality of Bread Supplemented with Mushroom Mycelia,” Food Chemistry, vol. 138, no. 1, pp. 70-76, 2023.
[CrossRef] [Google Scholar] [Publisher Link]
[18] Nigistie Ejigu et al., “Evaluation of Oyster Mushroom (Pleurotus Ostreatus) Production Using Water Hyacinth (Eichhornia Crassipes) Biomass Supplemented With Agricultural Wastes,” International Journal of Food Science, vol. 2022, no. 1, pp. 1-9, 2022.
[CrossRef] [Google Scholar] [Publisher Link]
[19] Sevda Kirbağ, and Mehmet Akyüz, “Nutritive Value of Edible Wild and Cultured Mushrooms,” Turkish Journal of Biology, vol. 23, no. 2, pp. 125-130, 2010.
[CrossRef] [Google Scholar] [Publisher Link]
[20] T. Jayakumar et al., “In-Vitro and In-Vivo Antioxidant Effects of the Oyster Mushrooms Pleurotus Ostreatus,” Food Research International, vol. 44, no. 4, pp. 851-861, 2011.
[CrossRef] [Google Scholar] [Publisher Link]
[21] Solomon P. Wasser, “Medicinal Mushroom Science: Current Perspectives, Advances, Evidence, and Challenges,” Medicinal Mushroom Science, vol. 37, no. 6, pp. 345-356, 2014.
[CrossRef] [Google Scholar] [Publisher Link]
[22] Otakar Rop, Jiri Mlcek, and Tunde Jurikova, “Beta-Glucans in Higher Fungi and Their Health Effects,” Nutrition Review, vol. 67, no. 11, pp. 624-631, 2009.
[CrossRef] [Google Scholar] [Publisher Link]
[23] Miriam Sari et al., “Screening of Beta-Glucan Contents in Commercially Cultivated and Wild-Growing Mushrooms,” Food Chemistry, vol. 216, pp. 45-51, 2017.
[CrossRef] [Google Scholar] [Publisher Link]
[24] Jörg Nitschke et al., “A New Colorimetric Method to Quantify β-1,3-1,6-Glucans in Comparison with Total β-1,3-Glucans in Edible Mushrooms,” Food Chemistry, vol. 127, no. 2, pp. 791-796, 2011.
[CrossRef] [Google Scholar] [Publisher Link]
[25] Kaisun Nesa Lesa et al., “Nutritional Value, Medicinal Importance, and Health-Promoting Effects of Dietary Mushrooms (Pleurotus Ostreatus),” Journal of Food Quality, vol. 2022, no. 1, pp. 1-9, 2022.
[CrossRef] [Google Scholar] [Publisher Link]
[26] Iwona Golak-Siwulska et al., “Bioactive Compounds and Medicinal Properties of Oyster Mushrooms (Pleurotus Sp.),” Folia Horticulturae, vol. 30, no. 2, pp. 191-201, 2018.
[CrossRef] [Google Scholar] [Publisher Link]
[27] William Horwitz, Official Methods of Analysis of AOAC International, 18th Ed, AOAC International, 2005.
[Google Scholar] [Publisher Link]
[28] Ronald O. Ocampo, and Normalina P. Usita, “Development of Lubeg (Syzygiumlineatum (Roxb.) Merr. & Perry) Processed Products,” Asia Pacific Journal of Multidisciplinary Research, vol. 3, no. 4, pp. 118-123, 2015.
[Google Scholar] [Publisher Link]
[29] Kumela D. Tolera, and Solomon Abera, “Nutritional Quality of Oyster Mushroom (Pleurotus Ostreatus) as Affected by Osmotic Pretreatments and Drying Methods,” Food Science Nutrition, vol. 5, no. 5, pp. 989-996, 2017.
[CrossRef] [Google Scholar] [Publisher Link]
[30] Mostak Ahmed, Noorlidah Abdullah, and M.M. Nuruddin, “Yield and Nutritional Composition of Oyster Mushrooms: An Alternative Nutritional Source for Rural People,” Malaysian Science, vol. 45, no. 11, pp. 1609-1615, 2016.
[Google Scholar] [Publisher Link]
[31] Mukundraj Govindrao Rathod et al., “Comparative Study of Nutritional Composition of Oyster Mushroom Pleurotus Florida Cultivated on Different Consortiums of Substrates,” World Journal of Advanced Engineering Technology and Sciences, vol. 8, no. 1, pp. 216-224, 2023.
[Google Scholar] [Publisher Link]
[32] Asma Irshad et al., “Determination of Nutritional and Biochemical Composition of Selected Pleurotus Spps,” BioMed Research International, vol. 2023, no. 1, pp. 1-10, 2023.
[CrossRef] [Google Scholar] [Publisher Link]
[33] L.C. Okpala, and P.N. Egwu, “Utilization of Broken Rice and Cocoyam Flour Blends in the Production of Biscuits,” Nigerian Food Journal, vol. 33, no. 1, pp. 8-11, 2015.
[CrossRef] [Google Scholar] [Publisher Link]
[34] S.S Kulkarni et al., “Development of Nutrient-Rich Noodles by Supplementation with Malted Ragi Flour,” International Food Research Journal, vol. 19, no. 1, pp. 309-313, 2012.
[Google Scholar] [Publisher Link]
[35] Ocheme Boniface Ocheme et al., “Proximate Composition, functional, and Pasting Properties of Wheat and Groundnut Protein Concentrate Flour Blends,” Food Science & Nutrition, vol. 6, no. 5, pp. 1173-1178, 2018.
[CrossRef] [Google Scholar] [Publisher Link]
[36] Numrah Nisar et al., “Proximate Composition, Functional Properties and Quantitative Analysis of Benzoyl Peroxide and Benzoic Acid in Wheat Flour Samples: Effect on Wheat Flour Quality,” PeerJ, vol. 2020, no. 8, pp. 1-19, 2020.
[CrossRef] [Google Scholar] [Publisher Link]
[37] Muhammad Javid Iqbal, Naureen Shams, and Kalsoom Fatima, Nutritional Quality of Wheat, Wheat - Recent Advance, 2022.
[CrossRef] [Google Scholar] [Publisher Link]
[38] Nasser Bahrman et al., “Differential Protein Expression was Assessed by Two-Dimensional Gel Electrophoresis for Two Wheat Varieties Grown at Four Nitrogen Levels,” Proteomics, vol. 4, no. 3, pp. 709-719, 2004.
[CrossRef] [Google Scholar] [Publisher Link]
[39] Masood Sadiq Butt et al., “Effect of Moisture and Packaging on the Shelf Life of Wheat Flour,” Internet Journal of Food Safety, vol. 4, pp. 1-6, 2004.
[Google Scholar] [Publisher Link]
[40] M. Atif Randhawa, Faqir M. Anjum, and Masood S. Butt, “Physico-Chemical and Milling Properties of New Spring Wheat Grown in Punjab and Sind for the Production of Pizza,” International Journal of Agriculture and Biology, vol. 4, no. 4, pp. 482-484, 2002.
[Google Scholar]
[41] Devin J. Rose, and Oscar A Pike, “A Simple Method to Measure Lipase Activity in Wheat and Wheat Bran as an Estimation of Storage Quality,” Journal of the American Oil Chemists' Society, vol. 83, pp. 415-419, 2006.
[CrossRef] [Google Scholar] [Publisher Link]
[42] K. Hossain et al., “Interdependence of Cultivar and Environment on Fiber Composition in Wheat Bran,” Australian Journal of Crop Science, vol. 7, no. 4, pp. 525-531, 2013.
[Google Scholar] [Publisher Link]
[43] Rana Das et al., “Physicochemical Properties and Sensory Evaluation of Sponge Cake Supplemented with Hot Air and Freeze Dried Oyster Mushroom (Pleurotus Sajor-Cajun),” World Journal of Engineering and Technology, vol. 8, no. 4, pp. 665-674, 2020.
[CrossRef] [Google Scholar] [Publisher Link]
[44] Rezoana Parvin et al., “Quality Improvement of Noodles with Mushroom Fortified and its Comparison with Local Branded Noodles,” NFS Journal, vol. 20, pp. 37-42, 2020.
[CrossRef] [Google Scholar] [Publisher Link]
[45] A.B. Nordiana et al., “The Effect of Oyster Mushroom (Pleurotus Sajor-Cajun) Flour Incorporation on the Physicochemical Quality and Sensorial Acceptability of Pasta,” International Food Research Journal, vol. 26, no. 4, pp. 1249-1257, 2019.
[Google Scholar] [Publisher Link]
[46] Pavel Prieto-Vázquez del Mercado, Luis Mojica, and Norma Morales-Hernández, “Protein Ingredients in Bread: Technological, Textural and Health Implications,” Foods, vol. 11, no. 16, pp. 1-18, 2022.
[CrossRef] [Google Scholar] [Publisher Link]
[47] Esmeralda Rangel-Vargas et al., “Edible Mushrooms as a Natural Source of Food Ingredient/Additive Replacer,” Foods, vol. 10, no. 11, pp. 1-19, 2021.
[CrossRef] [Google Scholar] [Publisher Link]
[48] C.M. Canja et al., “Dietary Fiber Role and Place in the Baking Product,” Bulletin of the Transilvania University of Braşov, vol. 9(58), no. 2, pp. 1-6, 2016.
[Google Scholar] [Publisher Link]
[49] D.D. Gallagher, and B.O. Schneeman, “Dietary Fiber,” Present Knowledge in Nutrition, 8th Ed, International Life Sciences Institute Press, 2001.
[Google Scholar] [Publisher Link]
[50] R. Lupton Joanne, “Microbial Degradation Products Influence Colon Cancer Risk: The Butyrate Controversy,” The Journal of Nutrition, vol. 134, no. 2, pp. 479-482, 2004.
[CrossRef] [Google Scholar] [Publisher Link]
[51] W.I.Wan Rosli, and M.S. Aishah, “Pleurotus Sajor-Cajun (PSC) Improves Nutrient Contents and Maintains the Sensory Properties of Carbohydrate-Based Products,” International Journal of Biological, Biomolecular, Agricultural, Food, and Biotechnological Engineering, vol. 6, no. 3, pp. 488-490, 2012.
[Google Scholar] [Publisher Link]
[52] Aneta Sławińska, Ewa Jabłońska-Ryś, and Anna Stachniuk, “High-Performance Liquid Chromatography Determination of Free Sugars and Mannitol in Mushrooms Using Corona-Charged Aerosol Detection,” Food Analytical Methods, vol. 14, no. 2, pp. 209-216, 2021.
[CrossRef] [Google Scholar] [Publisher Link]
[53] Atsushi Usami et al., “Characteristic Odorants from Bailingu Oyster Mushroom (Pleurotus Eryngii Var. Tuoliensis) and Summer Oyster Mushroom (Pleurotus Cystidiosus),” Journal of Oleo Science, vol. 63, no. 7, pp. 731-739, 2014.
[CrossRef] [Google Scholar] [Publisher Link]
[54] M.S Aishah, and W.I. Wan Rosli, “The Effect of the Addition of Oyster Mushroom (Pleurotus Sajor-Cajun) on Nutrient Composition and Sensory Acceptance of Selected Wheat-and Rice-Based Products,” International Food Research Journal, vol. 20, no. 1, pp. 183-188, 2013.
[Google Scholar] [Publisher Link]
[55] Fakhreddin Salehi, “Characterization of Different Mushroom Powder and Its Application in Bakery Products: A Review,” International Journal of Food Properties, vol. 22, no. 1, pp. 1375-1385, 2019.
[CrossRef] [Google Scholar] [Publisher Link]
[56] V.O Oyetayo, and R.R. Oyedeji, “Proximate and Mineral Composition of Bread Fortified with Mushroom (Plerotus Ostreatus and Calocybe Indica),” Microbiology Research Journal International, vol. 19, no. 4, pp. 1-9, 2017.
[CrossRef] [Google Scholar] [Publisher Link]