Hormonal Combinations, Genotypes and Explants Influence Regeneration Efficacy of Lentil
| International Journal of Agriculture & Environmental Science |
| © 2025 by SSRG - IJAES Journal |
| Volume 12 Issue 4 |
| Year of Publication : 2025 |
| Authors : Prosenjit Sarker, Gayatri Goswami, Priya Lal Biswas, Ujjal Kumar Nath |
10.14445/23942568/IJAES-V12I4P101
How to Cite?
Prosenjit Sarker, Gayatri Goswami, Priya Lal Biswas, Ujjal Kumar Nath, "Hormonal Combinations, Genotypes and Explants Influence Regeneration Efficacy of Lentil," SSRG International Journal of Agriculture & Environmental Science, vol. 12, no. 4, pp. 1-8, 2025. Crossref, https://doi.org/10.14445/23942568/IJAES-V12I4P101
Abstract:
Lentil is considered a vital vegetable worldwide and impacts the national economy. It ranks among the most nutritious legumes, with its seeds containing over 18% protein. The research focused on callus induction and regeneration ability of five lentil cultivars using various explants. The explants were cultured on MS medium mixture with various concentrations of plant hormones. Callus induction was recorded as 78.89% from cotyledons. Among the cultivars, Binamasur-6 produced the maximum callus (74.45%), while Binamasur-3 gave the maximum number of shoots (23) from the shoot tip. Callus induction was achieved at 72.45% when MS medium contained 1.0 mg/l 2,4-D. MS added with 2.0 mg/l BAP, 2.0 mg/l Kn and 1.0 mg/l NAA produced maximum shoots in Binamasur-3. The regenerated shoots of Binamasur-3 produced roots within 20 days when cultured on MS supplemented with 20 mg/l IBA. The survival rate of seedlings (53.13%) after hardening was highest in Binamasur-6. This study developed a regeneration protocol of lentil via callus production, which will aid in the selection of desired somaclonal variants and support genetic transformation or gene editing endeavors.
Keywords:
Lentil, Explant, Phytohormone, Callus induction, Somaclone.
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