Remediation of Mixed Heavy Metals using Acido-tolerant Bacterial co-cultures

International Journal of Agriculture & Environmental Science
© 2017 by SSRG - IJAES Journal
Volume 4 Issue 4
Year of Publication : 2017
Authors : Rosette Mathew, Veena Gayathri Krishnaswamy
pdf
How to Cite?

Rosette Mathew, Veena Gayathri Krishnaswamy, "Remediation of Mixed Heavy Metals using Acido-tolerant Bacterial co-cultures," SSRG International Journal of Agriculture & Environmental Science, vol. 4,  no. 4, pp. 40-49, 2017. Crossref, https://doi.org/10.14445/23942568/IJAES-V4I4P108

Abstract:

Background: Contaminated soils are common environmental problems throughout the world. Bioremediation is a process which helps to transfer the environment using microbes and alter the contaminants and alter the contaminants from a less hazardous to its original state within permissible limits. Objective: This present study focuses on the remediation of mixed heavy metals by acido–tolerant bacterial co–cultures. Methods: The removal of Heavy metals were analysed with UV spectroscopy and Atomic adsorption spectroscopy. Morphological identification by Scanning Electron Microscopy. The acido-tolerant cocultures were biochemically characterized and molecularly identified by 16 s r DNA Sequencing. Result: The isolated bacterial cocultures could remove mixed metals (Cr and Zn) at 50 mg/L concentration was observed to be 81% and 80.5% for Chromium and Zinc respectively. The Acido- tolerant bacterial co-cultures consisted of two strains, which were identified through biochemical tests and 16s RNA sequencing as Paracoccus.

Keywords:

Mixed Heavy Metals, Bioremediation, Acidotolerant, Co-cultures, Anthropogenic, Chromium, Zinc

References:

1) Abhijit Gupta, Jyoti Joia, Aditya Sood, Ridhi Sood, Candy Sidhu and Gaganjot Kaur. (2016) Microbes as Potential Tool for Remediation of Heavy Metals: A Review, Journal of Microbial & Biochemical Technology, 8:4.
2) Asha Latha, P., and S. Sandeep Reddy. (2013) Review on Bioremediation-Potential Tool for Removing Environmental Pollution International Journal of Basic and Applied Chemical Sciences: 2277-2073.
3) Ashwini M, Veena Gayathri Krishnaswamy and Nabila Fathima., (2016) Removal of chromium by a Bacterial consortium isolated from Kolar gold fields and chromium contaminated sites, Environment Conservation Journal 17(1&2) : 109-118
4) Benazir, J. Fathima, Suganthi R., Rajvel D., Padmini Pooja M and Mathithumilan B, (2010) Bioremediation of chromium in tannery effluent by microbial consortia. African Journal of Biotechnology 9.21: 3140-3143.
5) Beolchini, Francesca, Fonti V, Dell Anno A, Vegilo F, (2011) Bio-mobilization of heavy metals from contaminated sediments by acidophilic microbial consortia. Chemical Engineering Transactions 4: 833-888.
6) Bestawy, Ebtesam El, (2013) Bioremediation of heavy metalcontaminated effluent using optimized activated sludge bacteria. Applied water science 3(1): 181-192.
7) Brar, Satinder K., M. Verma; R. Y. Surampalli; K. Misra, R. D. Tyagi; N. Meunier; and J. F. Blais (2006) - Bioremediation of hazardous wastes—a review. Practice Periodical of Hazardous, Toxic, and Radioactive Waste Management 10(2): 59-72.
8) Calomiris, Jon J., John L. Armstrong, and Ramon J. Seidler, (1984) Association of metal tolerance with multiple antibiotic resistance of bacteria isolated from drinking water. Applied and Environmental Microbiology 47(6): 1238-1242
9) Deeb, Bahig El, and Abdullah D. Altalhi, (2009) Degradative plasmid and heavy metal resistance plasmid naturally coexist in phenol and cyanide assimilating bacteria. American J Biochem Biotechnol 5 (2): 84-93.
10) Dixit, Ruchita, et al. (2015) Bioremediation of heavy metals from soil and aquatic environment: an overview of principles and criteria of fundamental processes. Sustainability 7(2): 2189-2212.
11) Focardi, Silvia, Milva Pepi, and Silvano E. Focardi. (2013) Microbial reduction of hexavalent chromium as a mechanism of detoxification and possible bioremediation applications. Agricultural and biological sciences, biodegradation-life of science 12: 321-347.
12) Garbisu, C., and I. Alkorta. (2003) Basic concepts on heavy metal soil bioremediation. European Journal of Mineral Processing and Environmental Protection. 3(1): 58-66.
13) Gawali Ashruta, A.V.D. Nanoty, and U.K. Bhalekar (2014) Biosorption of heavy metals from aqueous solution using bacterial EPS. EPS 2 (4): 373-377.
14) Girma, Gosa, (2015) Microbial bioremediation of some heavy metals in soils: an update review. Indian Journal of Scientific Research 6(1): 147.
15) Greaney, Karen Marie, (2005) An assessment of heavy metal contamination in the marine sediments of Las Perlas Archipelago, Gulf of Panama. School of Life Sciences Heriot-Watt University, Edinburgh.
16) Jahani, Samaneh, Fatemi Faezeh, Ali Firoz-e-zare Mohammad, Reza Zolfaghar and Mohammad (2015), Isolation and characterization of Acidithiobacillus ferrooxidans strain FJS from Ramsar, Iran. Electronic Journal of Biology 11:4.
17) Jain, Akshata, T.H. Udayashankara, and Lokesh, K.S. (2014) Bioremediation of Heavy Metals using Mixed Bacterial Culture from contaminated Soil of Abandoned Kolar Gold Mine Residue. International Journal of Engineering Research and Technology. 3(8)
18) Jankaite, Audrone, and Saulius Vasarevičius. (2005) Remediation technologies for soils contaminated with heavy metals. Journal of environmental engineering and landscape management 13 (2): 109-113.
19) Kiran, K. (2012) Spectrophotometric determination of iron in water samples using 3-hydroxy benzyl amino benzoic acid. International Journal of Environmental Sciences 3(1): 20
20) Arpita Kulshreshtha, Ranu Agrawal, Manika Barar, Shilpi Saxena, (2014) A review on bioremediation of heavy metals in contaminated water. IOSR–J Environ Sci Toxicol Food Tech 8: 44-50.
21) Kumar, A., Bisht.B.S., Joshi.V.D. , Dhewa. T. (2011) Review on Bioremediation of Polluted Environment: A Management Tool. International Journal of Environmental Sciences 1(6): 1079.
22) Lambert, M., B. A. Leven, and R. M. Green, (2000) New methods of cleaning up heavy metal in soils and water. Environmental science and technology briefs for citizens. Kansas State University, Manhattan, KS.
23) Marques, Ana PGC, António OSS Rangel, and Paula ML Castro (2009) Remediation of heavy metal contaminated soils: phytoremediation as a potentially promising clean-up technology. Critical Reviews in Environmental Science and Technology 39 (8): 622-654.
24) Merina Paul Das and Neha Kumari., (2016) A Microbial Bioremediation Approach: Removal of Heavy Metal Using Isolated Bacterial Strains from Industrial Effluent Disposal Site. Int. J. Pharm. Sci. Rev. Res., 38(1), 19: 111-114.
25) Mishra, Debaraj, and Young Ha Rhee (2014) Microbial leaching of metals from solid industrial wastes. Journal of Microbiology 52 (1): 1-7.
26) Oliveira, Helena. Chromium as an environmental pollutant: insights on induced plant toxicity. Journal of Botany 2012 : (2012).
27) Paranthaman, S.R. and Karthikeyan, B. Bioremediation of paper mill effluent on growth and development of seed germination (Vigna mungo). CIBTech Journal of Biotechnology 2015 Vol. 4 (1) : 22-26.
28) QiuZhuo Zhang; Achal, V.; Xiang WeiNing; Wang DuanChao (2014) Identification of heavy metal resistant bacteria isolated from Yangtze River, China. International Journal of Agriculture and Biology 16 (3): 619-623.
29) Rampelotto, Pabulo Henrique. Extremophiles and extreme environments. (2013): 482-485.
30) Su, Chao (2014) A review on heavy metal contamination in the soil worldwide: situation, impact and remediation techniques. Environmental Skeptics and Critics 3(2): 24.
31) Subramanian, Sangeetha, Silviya Sam, and Gurunathan Jayaraman (2012) Hexavalent chromium reduction by metal resistant and halotolerant Planococcus maritimus VITP21. African Journal of Microbiology Research 47: 7339-7349.
32) Tripathi, M., and S. K. Garg (2010) Studies on selection of efficient bacterial strain simultaneously tolerant to hexavalent chromium and pentachlorophenol isolated from treated tannery effluent. Research Journal of Microbiology 5 (8): 707-716.
33) Vieira, Regine HSF, and Boya Volesky Biosorption (2010): a solution to pollution? International Microbiology 3 (1): 17- 24.
34) Wuana, Raymond A., and Felix E. Okieimen (2011) Heavy metals in contaminated soils: a review of sources, chemistry, risks and best available strategies for remediation." Isrn Ecology 2011, Article ID 402647.
35) Yang, Tao, Zheng Xu, Jiankang Wen and Limei Yang (2009) Factors influencing bioleaching copper from waste printed circuit boards by Acidithiobacillus ferrooxidans. Hydrometallurgy 97 (1): 29-32.
36) Zhitong Yaoa, Jinhui Lib, Henghua Xiec and Conghai Yuc, (2012) Review on remediation technologies of soil contaminated by heavy metals." Procedia Environmental Sciences 16: 722-729.