Screening of Halophilic Microorganisms (Oceanobacillus Oncorhynchi and Pseudomonas Stutzeri) for the Effect of Plant Growth Promotion and Its Formulation as a Biofertilizer

International Journal of Agriculture & Environmental Science
© 2018 by SSRG - IJAES Journal
Volume 5 Issue 2
Year of Publication : 2018
Authors : Deepalaxmi. R.K, Gayathri.C
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Deepalaxmi. R.K, Gayathri.C, " Screening of Halophilic Microorganisms (Oceanobacillus Oncorhynchi and Pseudomonas Stutzeri) for the Effect of Plant Growth Promotion and Its Formulation as a Biofertilizer ," SSRG International Journal of Agriculture & Environmental Science, vol. 5,  no. 2, pp. 11-19, 2018. Crossref, https://doi.org/10.14445/23942568/IJAES-V5I2P103

Abstract:

A quest for Plant Growth Promoting bacteria are increasing expeditiously as efforts are made to exploit them commercially as inoculum preparations ie., biofertilizers. In this work, a comparative study is carried out to assess the ability of the halobacterial strains in plant growth promotion and in addition tested for the ability of the isolate whether it could solubilize phosphate.These halobacterial strains were isolated and identified as Oceanobacillus oncorhynchi and Pseudomonas stutzeri by 16s rRNA sequence. The effectiveness of the isolate in plants seed germination and growth were analyzed in crops like Chickpea, Cowpea, Green gram and Pearl millet etc in which P.stutzeri showed a significant increase in plant root and shoot length than O.oncorhynchi. Thus, the ability to perform multifarious plant growth promoting activities together suggested uniqueness of P. stutzeri and its potential use in developing a cost-effective eco-friendly multifunctional biofertilizer and biocontrol agent in agriculture. Further the optimal growth conditions such as pH, NaCl concentrations and temperature were studied for the effective isolation of the isolate.

Keywords:

Plant Growth Promotingbacteria(PGPR), Biofertilizer, Oceanobacillus oncorhynchi, Pseudomonas stutzeri, Seed germination test

References:

1. Ahmad, F., Ahmad, I., & Khan, M. S. (2008). Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiological research, 163(2), 173-181.
2. Banerjee, M. R., & Yasmin, L. (2002). Sulfur oxidizing rhizobacteria: an innovative environment friendly soil biotechnological tool for better canola production. Proceeding of AGROENVIRON, 1-7.
3. Cappuccino, J. C., & Sherman, N. Microbiology: a laboratory manual. 1992. New York, 125-179.
4. Carlson, C. A., & Ingraham, J. L. (1983). Comparison of denitrification by Pseudomonas stutzeri, Pseudomonas aeruginosa, and Paracoccus denitrificans. Applied and Environmental Microbiology, 45(4), 1247-1253.
5. Cattelan, A. J., Hartel, P. G., & Fuhrmann, J. J. (1999). Screening for plant growth–promoting rhizobacteria to promote early soybean growth. Soil Science Society of America Journal, 63(6), 1670-1680.
6. Delgado‐García, M., Valdivia‐Urdiales, B., Aguilar‐González, C. N., Contreras‐Esquivel, J. C., & Rodríguez‐Herrera, R. (2012). Halophilic hydrolases as a new tool for the biotechnological industries. Journal of the Science of Food and Agriculture, 92(13), 2575- 2580.
7. Dey, R. K. K. P., Pal, K. K., Bhatt, D. M., & Chauhan, S. M. (2004). Growth promotion and yield enhancement of peanut (Arachis hypogaea L.) by application of plant growth-promoting rhizobacteria. Microbiological research, 159(4), 371- 394.
8. Dussault, H. P. (1955). An improved technique for staining red halophilic bacteria. Journal of bacteriology, 70(4), 484.
9. Glick, B. R., & Pasternak, J. J. (2003). Plant growth promoting bacteria. Molecular Biology-Principles and Applications of Recombinant DNA, 436-54.
10. Kennedy, I. R., Pereg-Gerk, L. L., Wood, C., Deaker, R., Gilchrist, K., & Katupitiya, S. (1997). Biological nitrogen fixation in non-leguminous field crops: facilitating the evolution of an effective association between Azospirillum and wheat. Plant and Soil, 194(1-2), 65-79.
11. Kloepper, J. W., & Schroth, M. N. (1978, August). Plant growth-promoting rhizobacteria on radishes. In Proceedings of the 4th international conference on plant pathogenic bacteria(Vol. 2, pp. 879-882).
12. Kumar, S., Karan, R., Kapoor, S., Singh, S. P., & Khare, S. K. (2012). Screening and isolation of halophilic bacteria producing industrially important enzymes. Brazilian Journal of Microbiology, 43(4), 1595-1603.
13. Patten, C. L., & Glick, B. R. (2002). Role of Pseudomonas putida indoleacetic acid in development of the host plant root system. Applied and environmental microbiology, 68(8), 3795-3801.
14. Ramadoss, D., Lakkineni, V. K., Bose, P., Ali, S., & Annapurna, K. (2013). Mitigation of salt stress in wheat seedlings by halotolerant bacteria isolated from saline habitats. SpringerPlus, 2(1), 6.
15. Richardson, A. E. (2001). Prospects for using soil microorganisms to improve the acquisition of phosphorus by plants. Functional Plant Biology, 28(9), 897-906.
16. Sawale, A. A., Kadam, T. A., & Mitkare, S. S. (2013). Isolation and Characterization of Secondary Metabolites from Halophilic Bacillus Species from Marin drive in Mumbai.
17. Saju, K. A., Babu, M. M., Murugan, M., & Raj, S. T. (2011). Survey on Halophilic microbial diversity of Kovalam Saltpans in Kanyakumari District and its industrial applications.
18. Tipre, S., Pindi, P. K., & Sharma, S. (2015). Biotechnological potential of a Halobacterium of family Bacillaceae.
19. Viveganandan, G., & Jauhri, K. S. (2000). Growth and survival of phosphate-solubilizing bacteria in calcium alginate. Microbiological research, 155(3), 205-207.