Epidemiological Studyof Entamoeba Histolytica Among People Living In Rural Area Nkombo Sector, Rusizi- Rwanda

International Journal of Medical Science
© 2020 by SSRG - IJMS Journal
Volume 7 Issue 5
Year of Publication : 2020
Authors : Fulgence Munyandamutsa, Dr. Arpita Sharma, Dr. Callixte Yadufasije, Dr. Joseph Mucumbitsi
pdf
How to Cite?

Fulgence Munyandamutsa, Dr. Arpita Sharma, Dr. Callixte Yadufasije, Dr. Joseph Mucumbitsi, "Epidemiological Studyof Entamoeba Histolytica Among People Living In Rural Area Nkombo Sector, Rusizi- Rwanda," SSRG International Journal of Medical Science, vol. 7,  no. 5, pp. 15-21, 2020. Crossref, https://doi.org/10.14445/23939117/IJMS-V7I5P105

Abstract:

Objective: The aim of the study was to assess the prevalence and risk factors associated to E. histolytica in patients attended Nkombo health center using direct microscopy examination (wet mount) for the identification of intestinal parasitic infections.
Method: All samples were tested with wet mount technique using light microscope (in saline water and iodine smears) for the determination of E. histolytica infection.
Results: The prevalence of E. histolytica by the help ofwet mount test was 44.89%. The prevalence of infection in males was 47/66 (31.97%), and was higher than that of females 19/66 (12.92%). The lowest prevalence of E. histolytica was detected in the extremities of age groups infants and elders with 13% and the highest prevalence of E. histolytica was observed in group of age 30-39 years old (21.21%). A prevalence of 19.69% was declared in participants usually practising fishing work and findings were significant (P < 0.005). Street food consumption and drinking of non-treated water were significantly the main source of infections with (P < 0.05).
Conclusion: Generally Entamoebahistolytica is quite taken into account as a public health problem at Nkombo health center. Moreover it is very important to conduct more studies to yield data on the source and risk factors to ameliorate the education and prevent public health threats to secure Nkombo people from all gastric intestinal parasites starting from E. histolytica.

Keywords:

Amebiasis, Patients, Parasite, Entamoebahystolytica, Nkombo

References:

[1] John CC, Salata RA. Amebiasis. In: Kliegman RM, Stanton B, St. Geme J, Schor N, Behrman RE, editors. Nelson textbook of pediatrics. Philadelphia: Saunders; 2010. p. 1123-5.
[2] Pham Duc P, Nguyen-Viet H, Hattendorf J, Zinsstag J, Dac Cam P, Odermatt P. Risk factors for Entamoebahistolytica infection in an agricultural community in Hanam province, Vietnam. Parasit Vectors. 2011. P. 4-102.
[3] Al-Harthi S, Jamjoom M. Diagnosis and differentiation of entamoeba infection in Makhah Al Mukarramah using microscopy and stool antigen detection kits. World J Med Sci. 2007. Vol. 2, 15-20.
[4] Ilikkan DY, Ilikkan B, Vural M. Amebiasis in infancy in the middle-high socioeconomic class in Istanbul, Turkey. Pediatr Infect Dis J. 2005, vol. 24, p. 929-30.
[5] Haque R., Mondal D, Duggal P, Kabir M, Roy S, Farr B.M, Sack R.B, and Petri W.A.
[6] Ryan, K.J, Ray, C.G. Sherris Medical Microbiology, An Introduction to infectious diseases. McGraw-Hill Medical Publishing Division, New York, 2004; 4:261-270.
[7] Seydel K.B., Stanley S.L. Jr. Entamoebahistolytica induces host cell death in amebicliver abscess by a non- Fas-dependent, nontumornecrosis factor alpha-dependent pathway of apoptosis. Infect Immun. 199
[8] Gunther J, Shafir S, Bristow B, Sorvillo F. Short report: amebiasis-related mortality among United States residents, 1990–2007. Am J Trop Med Hyg 2011.vol. 85, p. 1038–40.
[9] Ralston KS, Solga MD, Mackey-Lawrence NM, et al.. Trogocytosis by Entamoebahistolytica contributes to cell killing and tissue invasion. Nature. 2014. P. 508–30.
[10] Gahamanyi, Noel, Mugabo Jean De Dieu, Bayingana Claude, (2016). Prevalence OfEntamoebaHistolyticaIn Stool Specimens At Muhondo Health Center, Rwanda. Afr. J. Cln. Exper.Microbiol.17 (2): 83-87.
[11] Apurba S. S. (2014).Essentials of Medical Parasitology 1st Edition.Jaypee Brothers Medical Publishers.
[12] World Health Organization (2019). Bench aids for the diagnosis of intestinal parasites 2nd edition.
[13] World Health Organization. Basic Laboratory Methods in Parasitology. (1991): 25-28.
[14] Paniker, J. (2018). Textbook of medical parasitology 8th edition, Jaypee Brothers Medical Publishers (P) Ltd 4838/24, Ansari Road, Daryaganj.
[15] Sadaga GA, Kassem HH. Prevalence of intestinal parasites among primary schoolchildren in Derna District, Libya.J Egypt SocParasitol 2007;37:205-14.
[16] Rajasuriya K, Nagaratnam N. Hepatic amoebiasis in Ceylon. J Trop Med Hyg. 2009;65(7):165–78.
[17] De Silva N, De Silva H, Jayapani V. Intestinal parasitoses in the Kandy area, Sri Lanka. Southeast Asian J Trop Med Public Health.1994; 25:469.
[18] Singh A, Banerjee T, Kumar R, Shukla SK. Prevalence of cases of amebic liver abscess in a tertiary care Centre in India: a study on risk factors, associated microflora and strain variation of Entamoebahistolytica. PLoS One. 2019;14(4):e0214880.
[19] Gahamanyi N, Mugabo JD, Bayingana C. Prevalence of Entamoebahistolytica in stool specimens at Muhondo Health Center, Rwanda. African Journal of Clinical and Experimental Microbiology.2016;17(2):83-7. doi: 10.4314/ajcem.v17i2.2.
[20] Niyizurugero E, Ndayanze JB, Bernard K. Prevalence of intestinal parasitic infections and associated risk factors among Kigali Institute of Education students in Kigali, Rwanda. Trop Biomed. 2013;30(4):718-26.
[21] Espinosa-Cantellano M, Martinez-Palomo A. Pathogenesis of intestinal amebiasis: from molecules to disease. ClinMicrobiol Rev. 2000;13(2):318-31. doi: 10.1128/cmr.13.2.318- 331.2000.
[22] Alyousefi NA, Mahdy MA, Mahmud R, Lim YA. Factors associated with high prevalence of intestinal protozoan infections among patients in Sana’a City, Yemen. PLoS One. 2011; 6 (7):e22044. doi: 10.1371/journal.pone.0022044.
[23] Feachem RG, Bradley DJ, Garelick H, Mara DD. Sanitation and Disease: Health Aspects of Excreta and Wastewater Management. New York: John Wiley & Sons; 1983.
[24] Cavalini LT, Ponce de Leon ACM. Morbidity and mortality in Brazilian municipalities: a multilevel study of the association between socioeconomic  and healthcare indicators. International Journal of Epidemiology. 2008; 37:775–785.
[25] Costa-Cruz JM, Cardoso ML, Marques DE. Intestinal parasites in school food handlers in the City of Uberlandia (Minas Gerais), Brazil. Rev Institute Medical Tropical Soa Paulo. 1995;37:191–196.
[26] Idowu OA, Rowland SA. Oral fecal parasites and personal hygiene of food handlers in Abeokuta, Nigeria. African Health Science. 2006;6:160–164.
[27] Okamoto, M, T, Ohata K, Togo G, Hada T, Katamoto T, Amebic colitis in asymptomatic subjects with positive fecal occult blood test results: clinical features different from symptomatic cases. Am J Trop Med Hyg. 2005; 73:934–5.