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Viability and Tyrosinase Inhibition of Catechins Isolated from Uncaria gambir on B16F0 Cells


International Journal of Pharmacy and Biomedical Engineering
© 2023 by SSRG - IJPBE Journal
Volume 10 Issue 3
Year of Publication : 2023
Authors : Widyastuti
10.14445/23942576/IJPBE-V10I3P102
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How to Cite?

Widyastuti, "Viability and Tyrosinase Inhibition of Catechins Isolated from Uncaria gambir on B16F0 Cells," SSRG International Journal of Pharmacy and Biomedical Engineering, vol. 10,  no. 3, pp. 7-12, 2023. Crossref, https://doi.org/10.14445/23942576/IJPBE-V10I3P102

Abstract:

Catechins are found in various plants such as tea, gambier, apples, persimmons, chocolate, grapes and so on. Catechins are compounds that determine the quality of gambier. The content of catechins in gambier can reach more than 90%. Catechins isolated from gambier have not been tested for their activity. Research on the activity of catechins derived from Uncaria gambir, which has activity as an inhibitor tyrosinase, has not been widely carried out. This study aimed to test the catechins isolated from the Uncaria gambir plant against cytotoxic effects on B16F0 cells using an MTT assay with gambir catechin concentrations of 25-500 µg/ml. Furthermore, its activity against the tyrosinase enzyme was determined using mushroom tyrosinase. From the results obtained with the purity of catechins (C90 and C98), increasing the concentration and the length of the test time gave a significant difference in reducing the viability of B16F0 cells (p<0.05) with IC50 values of C90 and C98 after 72 hours of 171.995 and 204.486 µg/mL. The IC50 values for tyrosinase enzyme inhibition from C90 and C98 were 19.047 and 11.772 µg/mL, respectively. Gambier catechins have a depigmenting agent effect without being toxic to cells in vitro.

Keywords:

Melanogenesis, Flavonoids, Gambier, Cytotoxic B16F0 cell, Inhibit tyrosinase.

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