Justification of Profitable Test Kit for Microbiological Viewing of Antimicrobials in Rooster Eggs

International Journal of Veterinary Science
© 2015 by SSRG - IJVS Journal
Volume 1 Issue 1
Year of Publication : 2015
Authors : Dr.V.Manish kuptha, B.Ragavendiran Rao
How to Cite?

Dr.V.Manish kuptha, B.Ragavendiran Rao, "Justification of Profitable Test Kit for Microbiological Viewing of Antimicrobials in Rooster Eggs," SSRG International Journal of Veterinary Science, vol. 1,  no. 1, pp. 15-17, 2015. Crossref, https://doi.org/10.14445/24550868/IJVS-V1I1P105


The present study was conducted to appraise a profitable test kit for the qualitative screening of eggs submitted to our laboratory as a first step for the difficult of drug residues. Forty hens at the summit of lay were reserved for 4 weeks without administering any antibiotic. Eggs were then composed and tested for the deficiency of drug residues. Antibiotic-free birds were subsequently separated into two groups. One group was administered tetracycline for three days and the other assembly left as organize. Eggs were together daily for two weeks from both groups and hardened for tetracycline residues by means of the disc dispersal technique and a profitable test kit. Both methods detected the occurrence of drug residues in test eggs with the saleable test kit able to sense residues over a longer epoch up to day 10. The revise indicated that the profitable test kit perhaps will be used for the uncovering of drug residues predominantly when the aim is to screen large numbers of samples speedily. Though, it is not susceptible enough to detect drug residues at lower concentrations and may not be suitable for assenting difficult.
In some of the hens can be affected by the anti microbial in rooster egg.


Residue, qualitative screening, tetracycline.


1. I.O. Fagbamila, S.S. Ngulukun, S.S. Ardzard, N. Sati, O.T. Ajayi, P.I. Ankeli, Y. Akalusi, L. Okeke, L. Ikpa, M.O. Odugbo and M. Muhannnad, Validation of Commercial Test Kit for Microbiological Screening of Antimicrobials in Chicken Eggs, Research Journal of Veterinary Sciences 5 (3): 75-80, 2012.
2. Biswas, A.K., N. Kondaiah, A.S.R. Anjaneyulu and P.K. MandaI, 2010. Food safety concerns of pesticides, veterinary drug residues and mycotoxins in meat and meat products. Asian J. Anim. Sci., 4: 46-55.
3. Ferrini, A.M., V. Mannoni and P. Aureli, 2006. Combined plate microbial assay (CPMA): A 6-plate method for simultaneous first and second level screening of antibacterial residues in meat. Food Addit. Contam. A, 23: 16-24.
4. Hassan, S.A.A., S.A. Shaddad, I.E. EI-Tayeb, M.A. Orner, M.H. Al-Nazawi and A.M. Homeida, 2007. Detection of long-acting ox tetracycline residue levels in tissue of desert sheep following intramuscular injection. Int. J. Pharmacal., 3: 299-301.
5. Pikkemaat, M.G., P.P. Mulder, J.W. Elferink, A. de Cocq, M.W. Nielen and H.J. van Egmond, 2007. Improved microbial screening assay for the detection of quinolone residues in poultry and eggs. J. Food Additives Contaminants, 24: 842-850.
6. Shahid, M.A., M. Siddique, M. Abubakar, M.J. Arshed, M. Asif and A. Ahmad, 2007. Status of ox tetracycline residues in chicken meat in Rawalpindi/Islamabad area of Pakistan. Asia J. Poultry Sci., 1: 8-15.
7. Stead, S.L., M. Caldow, A. Sharma, H.M. Ashwin, M. Sharman, A. De-Rijk and J. Stark, 2007. New method for the rapid identification of tetracycline residues in foods of animal origin-using the premi-test in combination with a metal ion chelation assay. Food Additives Contam. 24: 583-589.